Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1984 Jun;75(2):369-71. doi: 10.1104/pp.75.2.369.
The activity of the first enzyme of the shikimate pathway, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase, is demonstrated in extracts of Daucus carota cells grown in suspension culture. Maximum specific enzyme activity is found midway through the logarithmic growth of the culture; cells in lag and stationary phases of growth have lower enzyme levels. The enzyme is activated by tyrosine and tryptophan. The extent of activation varies during cell growth.
在悬浮培养的胡萝卜细胞提取物中证明了莽草酸途径的第一个酶,即 3-脱氧-D-阿拉伯庚酮糖 7-磷酸合酶的活性。最大比酶活出现在培养物对数生长期的中间阶段;生长的迟滞期和静止期的细胞具有较低的酶水平。该酶被酪氨酸和色氨酸激活。在细胞生长过程中,激活程度会发生变化。