光和真菌激发子诱导欧芹（Petroselinum crispum L.）悬浮培养细胞中的5-磷酸脱氧阿拉伯庚酮糖酸合成酶信使核糖核酸
Light and Fungal Elicitor Induce 3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthase mRNA in Suspension Cultured Cells of Parsley (Petroselinum crispum L.).
作者信息
Henstrand J M, McCue K F, Brink K, Handa A K, Herrmann K M, Conn E E
机构信息
Department of Horticulture, Purdue University, West Lafayette, Indiana 47906.
出版信息
Plant Physiol. 1992 Feb;98(2):761-3. doi: 10.1104/pp.98.2.761.
Light and fungal elicitor induce mRNA encoding 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase in suspension cultured cells of parsley (Petroselinum crispum L.). The kinetics and dose response of mRNA accumulation were similar for DAHP synthase and phenylalanine ammonia-lyase (PAL). Six micrograms of elicitor from Phytophthora megasperma f. glycinia gave a detectable induction within 1 hour. Induction of DAHP synthase and PAL mRNAs by light was transient, reaching maximal levels at 4 hours and returning to pretreatment levels after 24 hours. Our data suggest that either light or fungal elicitor transcriptionally activate DAHP synthase. A coordinate regulation for key enzymes in the synthesis of primary and secondary metabolites is indicated.
光和真菌激发子可诱导欧芹（Petroselinum crispum L.）悬浮培养细胞中编码3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸（DAHP）合酶的mRNA的表达。DAHP合酶和苯丙氨酸解氨酶（PAL）的mRNA积累动力学和剂量反应相似。来自大豆疫霉（Phytophthora megasperma f. glycinia）的6微克激发子在1小时内即可产生可检测到的诱导作用。光对DAHP合酶和PAL mRNA的诱导是短暂的，在4小时达到最高水平，24小时后恢复到预处理水平。我们的数据表明，光或真菌激发子可转录激活DAHP合酶。这表明了对初级和次级代谢产物合成中的关键酶的协同调控。
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