创伤诱导茄科中莽草酸途径的第一酶。

Department of Horticulture, Purdue University, West Lafayette, IN 47907.

Proc Natl Acad Sci U S A. 1989 Oct;86(19):7370-3. doi: 10.1073/pnas.86.19.7370.

The first enzyme of the shikimate pathway, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15), is induced by wounding potato or tomato tissue. The increase in enzyme activity is associated with elevated amounts of the enzyme as determined by immunoblots. The specific wound-induced protein synthesis is preceded by an increase in the mRNA encoding this enzyme. The induced mRNA of potato tuber, leaf, and stem tissue is translated into a precursor polypeptide that is recognized by antibodies raised against the mature enzyme from tuber plastids. Wounding also induces mRNA encoding phenylalanine ammonia-lyase (EC 4.3.1.5), a key enzyme of plant secondary metabolism. The time courses for the induction of the two enzymes are similar, suggesting coordinate regulation for the biosynthesis of primary and secondary aromatic compounds.

莽草酸途径的第一个酶，3-脱氧-D-阿拉伯庚酮糖-7-磷酸合酶（EC 4.1.2.15），由马铃薯或番茄组织的创伤诱导。通过免疫印迹测定，酶活性的增加与酶含量的增加有关。特定的创伤诱导蛋白合成是由编码该酶的 mRNA 增加引起的。来自块茎质体的成熟酶的抗体识别的前体多肽。创伤还诱导编码苯丙氨酸解氨酶（EC 4.3.1.5）的 mRNA，苯丙氨酸解氨酶是植物次生代谢的关键酶。两种酶的诱导时间进程相似，表明初级和次级芳香化合物生物合成的协调调节。