Chereskin B M, Clement-Metral J D, Gantt E
Smithsonian Environmental Research Center, 12441 Parklawn Drive, Rockville, Maryland 20852-1773.
Plant Physiol. 1985 Mar;77(3):626-9. doi: 10.1104/pp.77.3.626.
Detergent preparations isolated from thylakoids of the red alga Porphyridium cruentum, in a sucrose, phosphate, citrate, magnesium chloride medium consist of phycobilisomes and possess high rates of photosystem II activity. Characterization of these particles shows that the O(2)-evolving activity is stable for several hours and the pH optimum is about 6.5 to 7.2. Response of the system to light, electron donors and acceptors, and inhibitors verify that the observed activity, measured both as O(2) evolution and 2,6-dichlorophenol-indophenol reduction, is due to photosystem II. Furthermore, photosystem II is functionally coupled to the phycobilisome in this preparation since green light, absorbed by phycobilisomes of P. cruentum, is effective in promoting both O(2) evolution and 2,6-dichlorophenol-indophenol reduction. Photosystem II activity declines when light with wavelengths shorter than 665 nm is removed. Both 3-(3,4-dichlorophenyl)-1,1-dimethylurea and atrazine inhibit photosystem II activity in this preparation, indicating that the herbicide binding site is a component of the photosystem II-phycobilisome particle.
从红球藻紫球藻的类囊体中分离得到的洗涤剂制剂，在蔗糖、磷酸盐、柠檬酸盐、氯化镁培养基中由藻胆体组成，并具有较高的光系统II活性。对这些颗粒的表征表明，放氧活性在数小时内稳定，最适pH约为6.5至7.2。该系统对光、电子供体和受体以及抑制剂的反应证实，观察到的活性（以放氧和2,6-二氯酚靛酚还原测量）是由于光系统II。此外，在该制剂中光系统II在功能上与藻胆体偶联，因为紫球藻的藻胆体吸收的绿光有效地促进了放氧和2,6-二氯酚靛酚还原。当去除波长小于665nm的光时，光系统II活性下降。3-(3,4-二氯苯基)-1,1-二甲基脲和莠去津均抑制该制剂中的光系统II活性，表明除草剂结合位点是光系统II-藻胆体颗粒的一个组成部分。
