二氯酚靛酚的光还原作用：由三洗叶绿体和亚叶绿体碎片催化的光系统 2 反应。
Photoreduction of 2,6-dichlorophenolindophenol by diphenylcarbazide: a photosystem 2 reaction catalyzed by tris-washed chloroplasts and subchloroplast fragments.
机构信息
Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387.
出版信息
Plant Physiol. 1969 Nov;44(11):1645-9. doi: 10.1104/pp.44.11.1645.
Abstract
The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplasts. By varying the concentration of tris buffer at pH 8.0 during an incubation period it is shown that the destruction of oxygen evolution activity is accompanied by a corresponding emergence of an ability to photooxidize diphenylcarbazide, as evidenced by absorbance changes due to diphenylcarbazide at 300 nm. The temperature-sensitive oxidation of diphenylcarbazide is inhibited by DCMU and by high ionic strengths. This activity appears to measure the primary photochemical reaction of photosystem 2.
摘要
二苯卡巴肼作为电子供体与三洗叶绿体或亚叶绿体片段的 2,6-二氯苯酚靛酚的光还原偶联，为叶绿体的光系统 2 提供了一种简单灵敏的测定方法。通过在孵育期间改变 pH8.0 的 tris 缓冲液的浓度，可以证明氧释放活性的破坏伴随着光氧化二苯卡巴嗪能力的相应出现，这可以通过 300nm 处二苯卡巴嗪的吸光度变化来证明。二苯卡巴嗪的温度敏感氧化受 DCMU 和高离子强度的抑制。这种活性似乎可以测量光系统 2 的初级光化学反应。
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本文引用的文献
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Light dependent oxygen metabolism of chloroplast preparations. III. Photooxidation of ascorbic acid.叶绿体制剂的光依赖型氧代谢。III. 抗坏血酸的光氧化作用。
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