Diner B A, Wollman F A
Eur J Biochem. 1980 Sep;110(2):521-6. doi: 10.1111/j.1432-1033.1980.tb04894.x.
Highly active photosystem-II particles were rapidly isolated using detergents and obtained in good yield from a mutant of the green alga Chlamydomonas reinhardtii. The particles are completely devoid of reaction centers of photosystem I, and of the secondary electron acceptor to photosystem II. They show: (a) a specific activity (delta A of C550/unit chlorophyll) 4--7 times that of the starting material and of spinach chloroplasts: (b) an antenna size of 40 to 50 chlorophyll molecules containing little light-harvesting chlorophyll a/b complex (chlorophyll a/chlorophyll b = 4--6.4); (c) a ratio of variable to dark-adapted fluorescence yield of up to 3. Further treatment of these particles by ion-exchange chromatography largely removes five proteins and further decreases the antenna size with little loss in primary photoactivity.
使用去污剂可快速分离出高活性的光系统II颗粒，并且从莱茵衣藻的一个突变体中能够高产获得这些颗粒。这些颗粒完全没有光系统I的反应中心以及光系统II的次级电子受体。它们表现出：(a) 比起始材料和菠菜叶绿体的比活性（C550的吸光度变化/单位叶绿素）高4至7倍；(b) 天线大小为40至50个叶绿素分子，几乎不含有光捕获叶绿素a/b复合物（叶绿素a/叶绿素b = 4至6.4）；(c) 可变荧光产量与暗适应荧光产量的比率高达3。通过离子交换色谱对这些颗粒进行进一步处理，可大量去除五种蛋白质，并进一步减小天线大小，而初级光活性几乎没有损失。
