Department of Plant and Soil Biology, University of California, Berkeley, California 94720.
Plant Physiol. 1985 Mar;77(3):705-11. doi: 10.1104/pp.77.3.705.
Changes in the amounts of thylakoid galactolipids and proteins were monitored for 96 hours following iron resupply to iron-deficient sugar beet (Beta vulgaris L. cv F58-554H1) plants. During this period of iron nutrition-mediated chloroplast development, the amount of galactolipid per leaf area increased linearly with time. Assuming galactolipids are an index for the amount of thylakoids, then there was a linear synthesis of thylakoid membranes during regreening. Total thylakoid protein synthesis, however, lagged behind galactolipid synthesis, suggesting that proteins are inserted secondarily into the galactolipid matrix of the thylakoid membrane during development.Iron deficiency caused an increase in the free chlorophyll band under the conditions of gel electrophoresis used. Of the chlorophyll proteins resolved, the chlorophyll protein associated with photosystem I was most diminished in iron-deficient tissue, and appeared to recover most rapidly. Changes in the light-harvesting chlorophyll proteins are also discussed.The number of polypeptides resolved by lithium dodecyl sulfate-polyacrylamide gel electrophoresis was higher in iron-deficient thylakoids. During regreening, the number of resolved polypeptides decreased.
在缺铁的糖甜菜（Beta vulgaris L. cv F58-554H1）植物接受铁补充后的 96 小时内，监测了类囊体半乳糖脂和蛋白质含量的变化。在这个铁营养介导的叶绿体发育期间，每叶面积的半乳糖脂含量随时间呈线性增加。假设半乳糖脂是类囊体数量的指标，那么在返绿过程中类囊体膜的合成呈线性。然而，总类囊体蛋白合成落后于半乳糖脂合成，表明在发育过程中蛋白质是次要地插入类囊体膜的半乳糖脂基质中的。在所用的凝胶电泳条件下，缺铁会导致叶绿素带的自由增加。在分离的叶绿素蛋白中，与光系统 I 相关的叶绿素蛋白在缺铁组织中减少最多，并且似乎恢复最快。还讨论了捕光叶绿素蛋白的变化。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的多肽数量在缺铁的类囊体中更高。在返绿过程中，分辨出的多肽数量减少。
