Department of Plant and Soil Biology, University of California, Berkeley, California 94720.
Plant Physiol. 1985 Jun;78(2):296-9. doi: 10.1104/pp.78.2.296.
Chlorophyll-protein complexes and electron transport activities were measured during iron nutrition-mediated chloroplast development in sugar beet (Beta vulgaris L. cv F58-554H1). Results showed that the chlorophyll-protein complexes associated with the reaction centers of photosystem I (CP1) and photosystem II (CPa) and the electron transport activities of these two photosystems per leaf area increased rapidly during the first 24 to 48 hours of iron resupply to iron-deficient sugar beet plants. Bulk chlorophyll and the amounts of light-harvesting chlorophyll-proteins increased after a lag period of 24 hours. The changes in chlorophyll-proteins with time were apparently the cause of an initial increase, then decrease, in the chlorophyll a/b ratio during iron resupply. There was evidence that iron deficiency diminished photosystem I more than photosystem II. We propose that there are two distinct phases in iron nutrition-mediated chloroplast development: (a) the commencement of the synthesis of the lipid matrix of the thylakoid membrane, including a fully functioning electron transport (and photosynthetic) system, during the first 24 hours of iron resupply; and (b) after 24 to 48 hours, the formation of the bulk of the thylakoid proteins, including the light-harvesting chlorophyll-proteins with which the large increase in total chlorophyll is associated.
在缺铁甜菜（Beta vulgaris L. cv F58-554H1）叶绿体发育过程中，研究了铁营养对叶绿素-蛋白复合物和电子传递活性的影响。结果表明，在缺铁甜菜植株重新供铁的前 24 至 48 小时内，与光系统 I（CP1）和光系统 II（CPa）反应中心相关的叶绿素-蛋白复合物以及这两个光系统的单位叶面积的电子传递活性迅速增加。大量叶绿素和光捕获叶绿素-蛋白的量在 24 小时的滞后期后增加。叶绿素-蛋白随时间的变化显然是重新供铁过程中叶绿素 a/b 比值先增加后减少的原因。有证据表明，缺铁对光系统 I 的影响大于光系统 II。我们提出，铁营养介导的叶绿体发育有两个明显的阶段：（a）在重新供铁的前 24 小时内，类囊体膜的脂质基质开始合成，包括一个功能齐全的电子传递（和光合作用）系统；（b）24 至 48 小时后，大量类囊体蛋白的形成，包括与总叶绿素大量增加相关的光捕获叶绿素-蛋白。
