从间断的聚蔗糖梯度中分离大豆叶片线粒体。
Isolation of mitochondria from soybean leaves on discontinuous percoll gradients.
机构信息
Plant Photobiology Lab, Plant Physiology Institute, United States Department of Agriculture/Agricultural Research Service, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705.
出版信息
Plant Physiol. 1985 Apr;77(4):1010-2. doi: 10.1104/pp.77.4.1010.
Abstract
A technique to isolate mitochondria from chamber-grown soybeans (Glycine max cv Williams) was developed. The mitochondria were isolated by centrifugation on discontinuous Percoll gradients which yielded a sharp band of mitochondria contaminated by only 4% of the total chlorophyll in the gradient. Contamination by peroxisomes was also slight. The isolated mitochondria oxidized malate plus glutamate, NADH, and malate with respiratory control. They also showed cyanide-insensitive, alternative pathway activity which was inhibited by salicylhydroxamic acid.
摘要
我们开发了一种从腔室培养的大豆（Glycine max cv Williams）中分离线粒体的技术。通过在不连续的 Percoll 梯度上离心，线粒体被分离出来，得到的线粒体带只有梯度中总叶绿素的 4%被污染。过氧化物酶体的污染也很小。分离的线粒体氧化苹果酸加谷氨酸、NADH 和苹果酸，具有呼吸控制。它们还表现出对氰化物不敏感的替代途径活性，该活性被水杨羟肟酸抑制。
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