Saini H S, Bassi P K, Spencer M S
Department of Plant Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
Plant Physiol. 1985 Apr;77(4):940-3. doi: 10.1104/pp.77.4.940.
Effects of ethylene, gibberellins, and kinetin on the germination of two lots of Chenopodium album L. seeds, collected from the field in 1982 and 1983, were studied in relation to the availability of nitrate. The experiments were conducted in darkness and at temperatures ranging from 12 to 32 degrees C. Ethylene induced over 75% germination in the 1983 seed but had little effect on the 1982 seed. Nitrate was only slightly promotive in either of the two seed lots. A combination of ethylene and nitrate, however, acted synergistically on 1982 seed, resulting in as much germination as that induced in 1983 seed by ethylene alone. In 1983 seed, a combination of ethylene and nitrate was only marginally more effective than ethylene. A similar relationship was observed in the effects of gibberellic acid(4+7) (GA(4+7)) and nitrate on seeds from the two lots. The 1982 seed, which responded synergistically to combinations of nitrate with ethylene or GA(4+7) was found to contain an extremely low endogenous level of nitrate as compared to 1983 seed. Thus, high levels of either endogenous or applied nitrate appeared to enhance the germination response to ethylene or GA(4+7).Kinetin had no effect on 1982 seed and only a small promotive effect on 1983 seed. There was no synergism between kinetin and nitrate in either of the seed lots.
研究了乙烯、赤霉素和激动素对1982年和1983年从田间采集的两批藜种子萌发的影响，并探讨了其与硝酸盐有效性的关系。实验在黑暗中进行，温度范围为12至32摄氏度。乙烯可使1983年的种子发芽率超过75%，但对1982年的种子影响不大。硝酸盐对这两批种子的促进作用都很微弱。然而，乙烯和硝酸盐的组合对1982年的种子具有协同作用，发芽率与单独使用乙烯处理1983年种子的发芽率相当。在1983年的种子中，乙烯和硝酸盐的组合仅比单独使用乙烯略微有效。在赤霉酸（4+7）（GA(4+7)）和硝酸盐对两批种子的影响中也观察到了类似的关系。与1983年的种子相比，1982年的种子对硝酸盐与乙烯或GA(4+7)的组合有协同反应，但其内源硝酸盐水平极低。因此，无论是内源还是外源的高浓度硝酸盐似乎都能增强种子对乙烯或GA(4+7)的发芽反应。激动素对1982年的种子没有影响，对1983年的种子只有轻微的促进作用。在这两批种子中，激动素和硝酸盐之间都没有协同作用。
