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1
Target molecular size of the red beet plasma membrane ATPase.红甜菜质膜ATP酶的目标分子大小
Plant Physiol. 1985 Jul;78(3):642-4. doi: 10.1104/pp.78.3.642.
2
Change in Target Molecular Size of the Red Beet Plasma Membrane ATPase during Solubilization and Reconstitution.红甜菜质膜ATP酶在增溶和重组过程中靶分子大小的变化
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9
Bacterial phytotoxin, syringomycin, induces a protein kinase-mediated phosphorylation of red beet plasma membrane polypeptides.细菌植物毒素，丁香菌肽，诱导甜菜细胞质膜多肽的蛋白激酶介导的磷酸化。
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本文引用的文献

1
Characterization of the solubilized plasma membrane ATPase of red beet.红甜菜可溶质膜 ATP 酶的特性。
Plant Physiol. 1984 Sep;76(1):26-30. doi: 10.1104/pp.76.1.26.
2
Effects of vanadate on the plasma membrane ATPase of red beet and corn.钒酸盐对红甜菜和玉米质膜 ATP 酶的影响。
Plant Physiol. 1984 Jul;75(3):586-91. doi: 10.1104/pp.75.3.586.
3
Evidence for a beta-Aspartyl Phosphate Residue in the Phosphorylated Intermediate of the Red Beet Plasma Membrane ATPase.证据表明在红甜菜质膜 ATP 酶磷酸化中间产物中有一个β-天冬氨酰磷酸残基。
Plant Physiol. 1983 Aug;72(4):1133-5. doi: 10.1104/pp.72.4.1133.
4
Characterization of a NO(3)-Sensitive H-ATPase from Corn Roots.玉米根中一种对硝酸盐敏感的H-ATP酶的特性分析
Plant Physiol. 1983 Jul;72(3):837-46. doi: 10.1104/pp.72.3.837.
5
Plasma membrane ATPase of red beet forms a phosphorylated intermediate.红甜菜质膜 ATP 酶形成磷酸化中间产物。
Plant Physiol. 1983 Mar;71(3):507-12. doi: 10.1104/pp.71.3.507.
6
Characterization of a k-stimulated adenosine triphosphatase associated with the plasma membrane of red beet.与红甜菜质膜相关的钾刺激型三磷酸腺苷酶的特性分析
Plant Physiol. 1983 Feb;71(2):350-5. doi: 10.1104/pp.71.2.350.
7
Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots.钒酸盐、钼酸盐和叠氮化物对玉米根膜结合ATP酶及可溶性磷酸酶活性的影响。
Plant Physiol. 1982 Nov;70(5):1335-40. doi: 10.1104/pp.70.5.1335.
8
Partial characterization of a phosphorylated intermediate associated with the plasma membrane ATPase of corn roots.玉米根质膜 ATP 酶磷酸化中间产物的部分特性
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6922-6. doi: 10.1073/pnas.79.22.6922.
9
Molecular weight of Na, K-ATPase approximated by the radiation inactivation method.用辐射失活法估算钠钾ATP酶的分子量。
Biochem Biophys Res Commun. 1967 Nov 30;29(4):588-92. doi: 10.1016/0006-291x(67)90526-8.
10
Size determination of an equilibrium enzymic system by radiation inactivation: theoretical considerations.通过辐射失活法测定平衡酶系统的大小：理论思考
Biochem J. 1982 Sep 1;205(3):477-83. doi: 10.1042/bj2050477.

红甜菜质膜ATP酶的目标分子大小

Target molecular size of the red beet plasma membrane ATPase.

作者信息

Briskin D P, Thornley W R, Roti-Roti J L

机构信息

Plant Biochemistry and Bioregulation Laboratory, United States Department of Agriculture-Agricultural Research Service, Utah State University, UMC 63, Logan, Utah, 84322.

出版信息

Plant Physiol. 1985 Jul;78(3):642-4. doi: 10.1104/pp.78.3.642.

DOI:10.1104/pp.78.3.642
PMID:16664298
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1064791/
Abstract

Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using gamma-ray radiation from a (137)Cs source. Inactivation of vanadate-sensitive ATPase activity by gamma-ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit of the red beet plasma membrane ATPase has been demonstrated to have a molecular weight of about 100,000 by dodecyl-sulfate gel electrophoresis following (32)P-phosphorylation, it is suggested that the native enzyme may exist, at least, as a dimer of catalytic subunits.

摘要

利用来自铯-137源的γ射线对红甜菜（Beta vulgaris L.）质膜ATP酶进行辐射失活处理。γ射线辐射使钒酸盐敏感的ATP酶活性失活，随着总剂量增加呈指数下降，表明单个靶标大小经计算分子量约为228,000。由于经32P磷酸化后通过十二烷基硫酸钠凝胶电泳已证明红甜菜质膜ATP酶的催化亚基分子量约为100,000，因此表明天然酶至少可能以催化亚基二聚体形式存在。