Briskin D P, Thornley W R, Roti-Roti J L
Plant Biochemistry and Bioregulation Laboratory, United States Department of Agriculture-Agricultural Research Service, Utah State University, UMC 63, Logan, Utah, 84322.
Plant Physiol. 1985 Jul;78(3):642-4. doi: 10.1104/pp.78.3.642.
Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using gamma-ray radiation from a (137)Cs source. Inactivation of vanadate-sensitive ATPase activity by gamma-ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit of the red beet plasma membrane ATPase has been demonstrated to have a molecular weight of about 100,000 by dodecyl-sulfate gel electrophoresis following (32)P-phosphorylation, it is suggested that the native enzyme may exist, at least, as a dimer of catalytic subunits.
利用来自铯-137源的γ射线对红甜菜（Beta vulgaris L.）质膜ATP酶进行辐射失活处理。γ射线辐射使钒酸盐敏感的ATP酶活性失活，随着总剂量增加呈指数下降，表明单个靶标大小经计算分子量约为228,000。由于经32P磷酸化后通过十二烷基硫酸钠凝胶电泳已证明红甜菜质膜ATP酶的催化亚基分子量约为100,000，因此表明天然酶至少可能以催化亚基二聚体形式存在。
