Department of Biological Sciences, Unit 1, Florida State University, Tallahassee, Florida 32306.
Plant Physiol. 1985 Oct;79(2):420-4. doi: 10.1104/pp.79.2.420.
Nanogram tissue samples from apical meristems of Sinapis alba were assayed for sucrose, total soluble hexosyl equivalents ( identical with glucose and fructose plus hexoses from sucrose hydrolysis), and total soluble glucosyl equivalents ( identical with glucose plus glucose from sucrose hydrolysis). On dry weight basis, sucrose concentration increased by more than 50% within 10 hours after the start of either a long photoperiod or a short photoperiod displaced by 10 hours in the 24-hour cycle (;displaced short day'). (These treatments induce flower initiation) Glucose and fructose concentrations were close to zero in vegetative meristems and remained low compared to sucrose in meristems of induced plants. Within a single meristem, the peripheral and the central zones had similar concentrations of sucrose. Our results indicate that an early physiological event in floral transition is the accumulation of sucrose in the meristem.
从白芥根尖分生组织中提取纳克级组织样本，用于检测蔗糖、总可溶性己糖当量（与葡萄糖和果糖以及蔗糖水解产生的己糖相同）和总可溶性葡糖糖当量（与葡萄糖和蔗糖水解产生的葡萄糖相同）。在开始长日照或短日照 10 小时后，24 小时周期中被推迟 10 小时的（“移位短日”），10 小时内，蔗糖浓度增加了 50%以上。（这些处理诱导开花）在营养分生组织中，葡萄糖和果糖浓度接近零，并且与诱导植物的分生组织中的蔗糖相比，葡萄糖和果糖浓度仍然较低。在单个分生组织中，外围区和中央区的蔗糖浓度相似。我们的结果表明，花发育过程中的早期生理事件是在分生组织中积累蔗糖。
