Laboratoire de Physiologie Végétale and Centre de Physiologie Végétale Appliquée (IRSIA), Département de Botanique, Université de Liège, Sart Tilman, B4000-Liege, Belgium.
Plant Physiol. 1977 Aug;60(2):282-5. doi: 10.1104/pp.60.2.282.
Results of previous investigations indicated that one of the early and essential events occurring in the apical meristem of Sinapis alba L. during the transition to flowering is the release to mitosis of the G(2) nuclei; the trigger to mitosis is generated in the leaves and its movement out of the leaves begins around 16 hours after the start of the inductive treatment. The mitotic wave in the meristem culminates 10 hours later.In this paper, it is shown that a single application of a cytokinin (benzyladenine or zeatin) at concentrations ranging from 1 to 20 mug/ml directly to the apical bud of vegetative plants, at a time corresponding to the time of movement of the mitotic trigger in induced plants, produces a mitotic wave which is very similar to that found in induced plants. It is thus proposed that the mitotic component of the floral stimulus in Sinapis is a cytokinin. As the cytokinins are completely unable to induce flowering, it appears that there is a multicomponent floral stimulus in this species.
先前的研究结果表明，在甘蓝型油菜顶端分生组织向花转变的过程中，最早和必不可少的事件之一是 G2 核进入有丝分裂；有丝分裂的触发信号产生于叶片，大约在诱导处理开始后 16 小时，该信号开始从叶片输出。10 小时后，顶端分生组织中的有丝分裂波达到顶峰。本文表明，在诱导植物中，有丝分裂触发信号移动的时间点，将细胞分裂素（苄基腺嘌呤或玉米素）以 1 至 20 微克/毫升的浓度直接应用于营养植物的顶端芽，会产生与诱导植物中发现的非常相似的有丝分裂波。因此，拟南芥中花刺激的有丝分裂成分是细胞分裂素。由于细胞分裂素完全不能诱导开花，因此在该物种中存在多成分花刺激。
