University of Hawaii NifTAL Project, Paia, Hawaii 96779.
Plant Physiol. 1985 Oct;79(2):562-3. doi: 10.1104/pp.79.2.562.
Using a split-root technique, roots of soybean plants were divided between two pots. In one of the two pots, two maize plants were grown and half of those pots were inoculated with the vesicular arbuscular mycorrhizal (VAM) fungus, Glomus fasciculatus. Fifty-two days after planting, (15)N-labeled ammonium sulfate was applied to the pots which contained only soybean roots. Forty-eight hours after application, significantly higher values for atom per cent (15)N excess were found in roots and leaves of VAM-infected maize plants as compared with the non-VAM-infected maize plants. Results indicated that VAM fungi did enhance N transfer from one plant to another.
采用分根技术,将大豆植株的根系分开种植在两个盆中。在其中一个盆中,种植了两株玉米,并对其中一半的盆进行了泡囊丛枝菌根(VAM)真菌,即聚丛球囊霉的接种。种植 52 天后,仅含有大豆根系的盆中施加了(15)N 标记的硫酸铵。施加后 48 小时,与未感染 VAM 的玉米植株相比,感染 VAM 的玉米植株的根和叶中的(15)N 过剩原子百分率明显更高。结果表明,VAM 真菌确实增强了氮从一株植物向另一株植物的转移。