Department of Plant Physiology, University of Groningen, P. O. Box 14, 9750 AA Haren (Gn) The Netherlands.
Plant Physiol. 1985 Nov;79(3):756-9. doi: 10.1104/pp.79.3.756.
Lipid and fatty acid composition of chloroplast thylakoid membranes was determined in two varieties of wheat (Triticum aestivum L.), the hardy Miranovskaja and the sensitive Penjamo. Plants were grown at room temperature or under frost hardening conditions (1.5 degrees C). Changes in lipid and fatty acid composition of the isolated thylakoids could be related to the temperature dependence of light-stimulated proton uptake. Changes in the thylakoid phospholipids upon hardening of the two varieties did not show any direct relation with low temperature tolerance of light-dependent H(+) uptake; neither did changes in phospholipid fatty acid chain lengthening to 20 and 22 C-atoms in combination with increased desaturation up to 6 double bonds. Increased low temperature tolerance of light-induced H(+) uptake by hardening was correlated with the following glycolipid changes: maintained glycolipid level, a proportionally increased digalactosyl diglyceride fraction, a decrease in thylakoid monogalactosyl diglyceride, increased sulfolipid fatty acid chain lengthening (20 and 22 C-atoms), and increased sulfolipid desaturation (4-6 double bonds). We suggest that the above mentioned changes in glycolipids have adaptive value for low temperature tolerance of light-dependent proton uptake.
叶绿体类囊体膜的脂质和脂肪酸组成在两种小麦（Triticum aestivum L.）品种中进行了测定，分别是耐寒的 Miranovskaja 和敏感的 Penjamo。植物在室温或霜冻硬化条件下（1.5 摄氏度）生长。分离的类囊体脂质和脂肪酸组成的变化可能与光刺激质子摄取的温度依赖性有关。两种品种硬化时类囊体磷脂的变化与光依赖性 H(+)摄取的低温耐受性没有直接关系；磷脂脂肪酸链延长到 20 和 22 个碳原子并结合增加的 6 个双键的不饱和度也没有关系。通过硬化增加光诱导 H(+)摄取的低温耐受性与以下糖脂变化有关：保持糖脂水平、二半乳糖基二甘油酯比例增加、类囊体单半乳糖基二甘油酯减少、硫脂脂肪酸链延长（20 和 22 个碳原子）和硫脂去饱和增加（4-6 个双键）。我们认为，糖脂的上述变化对于光依赖性质子摄取的低温耐受性具有适应性价值。
