Canada Department of Agriculture Research Station, Ste-Foy, Quebec, GIV 2J3, Canada.
Plant Physiol. 1975 Feb;55(2):356-9. doi: 10.1104/pp.55.2.356.
Lipids were labeled with (33)P during frost hardening of two varieties of winter wheat (Triticum aestivum), hardy Kharkov and much less hardy Champlein. The main labeled compounds were phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylglycerol. With time of incorporation the proportion of the radioactivity incorporated into the lipids increased in phosphatidylcholine, especially in Kharkov and at 1 C. During hardening, phospholipid synthesis was greatly stimulated in Kharkov, but much less in Champlein. The proportion of the phospholipids synthesized changed only little with hardening, with a trend towards an increase in phosphatidylcholine. Increased phospholipid synthesis does not seem to be a prerequisite to hardening in winter wheat. However, a high rate of phospholipid synthesis may be required to maintain frost resistance.
在冬小麦（Triticum aestivum）两个品种（耐寒的哈尔科夫和不太耐寒的尚普兰）的抗冻硬化过程中，用（33）P 对脂质进行了标记。主要标记化合物为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰甘油。随着掺入时间的增加，特别是在 1°C 时，Kharkov 中掺入脂质的放射性比例增加。在硬化过程中，Kharkov 中的磷脂合成大大受到刺激，但在 Champlein 中则较少。随着硬化的进行，合成的磷脂比例变化不大，趋向于增加磷脂酰胆碱。增加的磷脂合成似乎不是冬小麦硬化的先决条件。然而，维持抗冻性可能需要较高的磷脂合成速率。
