Section of Plant Biology, Cornell University, Ithaca, New York 14853.
Plant Physiol. 1985 Nov;79(3):856-61. doi: 10.1104/pp.79.3.856.
The subcellular distribution of the enzymes of de novo pyrimidine nucleotide biosynthesis was investigated in pea (Pisum sativum L. cv Progress No. 9) leaves. Aspartate carbamoyltransferase, the committed step of the pathway, was found to be strictly confined to the chloroplasts. Dihydro-orotase, orotate phosphoribosyl transferase, and orotidine decarboxylase activities were also found only in the plastids. The remaining enzyme of the pathway, dihydroorotate dehydrogenase, was shown to be mitochondrial.
我们研究了豌豆（Pisum sativum L. cv Progress No. 9）叶片中新嘧啶核苷酸生物合成途径的酶的亚细胞分布。该途径的关键步骤天冬氨酸氨甲酰基转移酶被严格局限在叶绿体中。二氢乳清酸酶、乳清酸磷酸核糖基转移酶和乳清酸脱羧酶的活性也只存在于质体中。该途径的最后一种酶，二氢乳清酸脱氢酶，被证明是线粒体的。
