Department of Biochemistry, La Trobe University, Bundoora, Victoria, 3083, Australia.
Plant Physiol. 1985 Dec;79(4):968-72. doi: 10.1104/pp.79.4.968.
The two soluble Ca(2+)-dependent protein kinases resolved from wheat (Triticum aestivum) embryo (protein kinases I and II) are inhibited by the phenothiazine-derived calmodulin antagonists trifluoperazine fluphenazine, and chlorpromazine. Protein kinases I and II are also inhibited by a variety of other calmodulin antagonists (including calmidazolium, amitriptyline, and iprindole), phosphodiesterase inhibitors (including flufenamic acid and papavarine) and by lanthanides. A number of compounds that inhibit mammalian Ca(2+) - and phospholipid-activated protein kinase (protein kinase C) including quercetin, polymixin B sulfate, and polyamines (as well as phenothiazine derivatives) also inhibit protein kinases I and II. Poly-l-lysine and poly-l-ornithine activate both plant Ca(2+)-dependent protein kinases.
从小麦(Triticum aestivum)胚中分离得到的两种可溶性 Ca(2+)-依赖性蛋白激酶(蛋白激酶 I 和 II)被苯并噻嗪衍生的钙调蛋白拮抗剂三氟拉嗪、氟奋乃静和氯丙嗪抑制。蛋白激酶 I 和 II 也被多种其他钙调蛋白拮抗剂(包括 calmidazolium、阿米替林和 iprindole)、磷酸二酯酶抑制剂(包括 flufenamic acid 和罂粟碱)和镧系元素抑制。许多抑制哺乳动物 Ca(2+) 和磷脂激活蛋白激酶(蛋白激酶 C)的化合物,包括槲皮素、多粘菌素 B 硫酸盐和多胺(以及苯并噻嗪衍生物),也抑制蛋白激酶 I 和 II。聚赖氨酸和聚鸟氨酸激活植物 Ca(2+)-依赖性蛋白激酶。