Morishita Y, Akogyeram C, Deu B, Criss W E
Biochim Biophys Acta. 1983 Feb 22;755(3):358-62. doi: 10.1016/0304-4165(83)90238-6.
Polyamine-responsive protein kinase, a cyclic nucleotide-independent protein kinase from the cytosol of Morris hepatoma 3924A, was stimulated 8-9 fold by several different polymers of polylysine, polyornithine and random copolymers of lysine-alanine; spermidine, spermine, and mixtures of spermine and spermidine stimulated 2, 3, and 5 fold, respectively. The protein kinase was not stimulated by poly-carboxybenzyl-lysine, random copolymer of lysine-tyrosine, polyhistidine, polymethionine, polyglutamic acid, polyaspartic acid, dipeptide (Lys-Lys), lysine, ornithine, and putresine. The polyamine stimulation of the protein kinase was prevented by certain specific charged carbohydrates: heparin, chondroitin sulfates A, B, and C, dextran sulfate and hyaluronic acid. It was not prevented by noncharged carbohydrates: dextran, glycogen, starch, sucrose, etc; or by sulfate salts: ammonium sulfate, potassium sulfate, sodium thiosulfate, etc. The inhibition was reversed by increased polylysine. Heparin was non-competitive inhibitor of Mg2+-ATP. It would appear that this enzyme is regulated by certain highly specific molecules with certain sizes and charges; plus charge is stimulatory, negative charge prevents the stimulation.
多胺反应性蛋白激酶是一种来自莫里斯肝癌3924A细胞溶质的不依赖环核苷酸的蛋白激酶，它被几种不同的聚赖氨酸、聚鸟氨酸以及赖氨酸 - 丙氨酸的无规共聚物刺激了8 - 9倍；亚精胺、精胺以及精胺和亚精胺的混合物分别刺激了2倍、3倍和5倍。该蛋白激酶不受聚羧苄基赖氨酸、赖氨酸 - 酪氨酸无规共聚物、聚组氨酸、聚甲硫氨酸、聚谷氨酸、聚天冬氨酸、二肽（Lys - Lys）、赖氨酸、鸟氨酸和腐胺的刺激。某些特定的带电荷碳水化合物可阻止多胺对该蛋白激酶的刺激：肝素、硫酸软骨素A、B和C、硫酸葡聚糖和透明质酸。不带电荷的碳水化合物：葡聚糖、糖原、淀粉、蔗糖等，或硫酸盐：硫酸铵、硫酸钾、硫代硫酸钠等则不能阻止。增加聚赖氨酸可逆转这种抑制作用。肝素是Mg2 + - ATP的非竞争性抑制剂。看来这种酶受某些具有特定大小和电荷的高度特异性分子调节；正电荷具有刺激作用，负电荷则阻止刺激。
