Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1986 Feb;80(2):578-82. doi: 10.1104/pp.80.2.578.
Combustion-based studies of the carbon-13 content of plants give only an integrated, long-term value for the isotope fractionation associated with photosynthesis. A method is described here which permits determination of this isotope fractionation in 2 to 3 hours. To accomplish this, the plant is enclosed in a glass chamber, and the quantity and isotopic content of the CO(2) remaining in the atmosphere are monitored during photosynthesis. Isotope fractionation studies by this method give results consistent with what is expected from combustion studies of C(3), C(4), and Crassulacean acid metabolism plants. This method will make possible a variety of new studies of environmental and species effects in carbon isotope fractionation.
基于燃烧的植物碳-13 含量研究只能给出与光合作用相关的同位素分馏的综合、长期值。本文描述了一种可在 2 到 3 小时内确定这种同位素分馏的方法。为此,将植物封入一个玻璃室中,并在光合作用过程中监测剩余大气中 CO(2)的数量和同位素含量。通过这种方法进行的同位素分馏研究结果与对 C(3)、C(4)和景天酸代谢植物的燃烧研究的预期结果一致。这种方法将使对环境和物种效应对碳同位素分馏的影响进行各种新的研究成为可能。