能够进行光合自养生长的培养大豆品系中的质体DNA含量。
Plastid DNA content in a cultured soybean line capable of photoautotrophic growth.
作者信息
Cannon G, Heinhorst S, Weissbach A
机构信息
Department of Cell Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
出版信息
Plant Physiol. 1986 Feb;80(2):601-3. doi: 10.1104/pp.80.2.601.
Abstract
The levels of chloroplast DNA in a cultured photoautotrophic soybean (Glycine max [L.] Merr. v Corsoy) cell line were determined by molecular hybridization. The cells were also grown photomixotrophically and heterotrophically as suspension cultures and the level of plastid DNA was found to be constant at approximately 26% of the total cellular DNA in all three growth modes. By comparison, total cellular DNA extracted from plants of the same variety used as the explant source for the cultured cells contained 12.3 to 18.9% (leaves and seeds) and 6.1 to 8.9% (roots) plastid DNA.
摘要
通过分子杂交测定了一种光合自养大豆（Glycine max [L.] Merr. v Corsoy）细胞系中叶绿体DNA的水平。这些细胞还以悬浮培养的方式进行光合混合营养生长和异养生长，发现质体DNA的水平在所有三种生长模式下均恒定，约占细胞总DNA的26%。相比之下，从用作培养细胞外植体来源的同一品种植物中提取的细胞总DNA含有12.3%至18.9%（叶片和种子）和6.1%至8.9%（根）的质体DNA。
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本文引用的文献
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