Vegetable Crops Department, IFAS, University of Florida, Gainesville, Florida 32611.
Plant Physiol. 1986 Mar;80(3):609-11. doi: 10.1104/pp.80.3.609.
To characterize the movement of sugars during kernel development in maize, a newly devised in vitro kernel development scheme was utilized. Viable seeds of wild type maize (Zea mays L.) as well as the mutant shrunken-2 (sh2) were found to mature when grown in culture with reducing sugars or sucrose as the carbon source. However, wild type and sh2 kernels had greater germination, starch content, and seed weight when sucrose, rather than reducing sugars, was the carbon source. By the use of labeled sucrose it was shown that sucrose can move into endosperm tissue without intervening degradation and resynthesis. These results show that when grown in vitro the maize seed can utilize reducing sugars for development, but it prefers sucrose.
为了研究玉米子粒发育过程中糖的运动情况，我们采用了一种新设计的体外子粒发育体系。在含有还原糖或蔗糖作为碳源的培养液中，野生型玉米（Zea mays L.）以及突变体 shrunken-2（sh2）的活种子都能正常成熟。然而，当以蔗糖而不是还原糖作为碳源时，野生型和 sh2 型玉米子粒的发芽率、淀粉含量和种子重量更大。通过使用标记的蔗糖，我们发现蔗糖可以在不经过降解和重新合成的情况下进入胚乳组织。这些结果表明，在体外培养条件下，玉米种子可以利用还原糖进行发育，但它更喜欢蔗糖。
