Biological Sciences Department, California State Polytechnic University, Pomona, California 91768.
Plant Physiol. 1986 Mar;80(3):790-1. doi: 10.1104/pp.80.3.790.
Segments (2.5 cm) cut from 3-day-old seedling roots of radish (Raphanus sativus L. ;Scarlet Globe') were cultured in medium with or without indoleacetic acid (IAA). Lateral root primordia frequency, determined for the central centimeter of segments, was dependent on IAA concentration and on conditions affecting IAA uptake. Dimethyl sulfoxide treatment, or a relatively low medium pH, greatly enhanced the response to exogenous IAA. It was concluded that a permeation barrier exists between the external medium and the hormone responsive sites within the radish seedling root.
从 3 天大的萝卜（Raphanus sativus L.；Scarlet Globe'）幼苗根中切下的片段（2.5 厘米）在含有或不含有吲哚乙酸（IAA）的培养基中进行培养。在片段的中央厘米处确定侧根原基的频率，其取决于 IAA 浓度和影响 IAA 摄取的条件。二甲基亚砜处理或相对较低的培养基 pH 值极大地增强了对外源 IAA 的反应。因此得出结论，在萝卜幼苗根的外部介质和激素反应部位之间存在渗透屏障。
