Department of Botany, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1986 May;81(1):186-91. doi: 10.1104/pp.81.1.186.
Hexokinase was measured by quantitative histochemical techniques in the apical meristem, primordia, and leaves of Dianthus chinensis L. The structural stages of development in the leaves sampled were determined by light and scanning electron microscopy. The results showed that activity decreased from the youngest primordia (1500 millimoles per kilogram dry weight per hour) to the mature leaves (200 millimoles per kilogram dry weight per hour) and that an intermediate leaf, the fourth youngest, showed the same declining pattern from its base to its tip. Surface views and measurements of these leaves revealed their basipetal maturation as seen by cell size, stomatal development, trichome differentiation, cuticular appearance, and leaf thickness. The intermediate leaf showed features representative of several stages in structural differentiation. It was concluded that the changes in hexokinase activity among the leaves of a shoot and within an individual leaf are similar and correlate with the degree of structural differentiation of the leaves.
用定量组织化学技术测定了中国石竹的顶端分生组织、原基和叶片中的己糖激酶。通过光镜和扫描电子显微镜观察了所取叶片的发育结构阶段。结果表明，活性从最年轻的原基（1500 毫摩尔/千克干重/小时）下降到成熟叶片（200 毫摩尔/千克干重/小时），第四年轻的中间叶片从其基部到顶端呈现出相同的下降模式。这些叶片的表面观察和测量显示，细胞大小、气孔发育、毛状体分化、角质层外观和叶片厚度表明它们是向基成熟的。中间叶片表现出结构分化的几个阶段的特征。结论是，一个芽的叶片之间和单个叶片内的己糖激酶活性变化相似，并与叶片的结构分化程度相关。
