保卫细胞中氨酰化步骤的酶和底物基础。
Enzymic and substrate basis for the anaplerotic step in guard cells.
机构信息
Departments of Biology and Pharmacology (1137), Washington University Division of Biology and Biomedical Sciences, Saint Louis, Missouri 63130.
出版信息
Plant Physiol. 1978 Oct;62(4):648-52. doi: 10.1104/pp.62.4.648.
Abstract
From the maximum rate of malate accumulation in Vicia faba L. guard cells during stomatal opening the maximum rate of organic anion synthesis is calculated to be 200 millimoles per kilogram dry weight per hour. A minimum estimate for the phosphoenolpyruvate (PEP) carboxylase-catalyzed reaction in guard cells is 650 millimoles per kilogram dry weight per hour which is significantly higher than in any other leaf tissue. The apparent K(mpep) of the guard cell enzyme is 60 mum at pH 8.7, but is probably higher at lower pH. The concentration of PEP in guard cells was 270mum (=2.2 x 10(-15) moles/guard cell pair) during anion synthesis. These results support the possibility that the carboxylation of PEP is the anaplerotic step in guard cells.
摘要
从蚕豆保卫细胞在气孔开放时积累苹果酸的最大速率计算，有机阴离子合成的最大速率为每小时每公斤干重 200 毫摩尔。对保卫细胞中磷酸烯醇丙酮酸（PEP）羧化酶催化反应的最小估计值为每小时每公斤干重 650 毫摩尔，这明显高于任何其他叶片组织。保卫细胞酶的表观 K(mpep)在 pH8.7 时为 60μm，但在较低 pH 值时可能更高。在阴离子合成过程中，保卫细胞中的 PEP 浓度为 270μm（=2.2×10^(-15)摩尔/保卫细胞对）。这些结果支持了 PEP 羧化作用是保卫细胞中氨同化作用的补料步骤的可能性。
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本文引用的文献
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Photosynthetic Carbon Metabolism in the Palisade Parenchyma and Spongy Parenchyma of Vicia faba L.蚕豆叶栅栏薄壁组织和海绵薄壁组织中的光合碳代谢
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Carbon dioxide metabolism in leaf epidermal tissue.叶片表皮组织中的二氧化碳代谢。
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