植物血球凝集素的高甘露寡糖连接到天冬酰胺 12 位，修饰后的寡糖连接到天冬酰胺 60 位。
The high mannose oligosaccharide of phytohemagglutinin is attached to asparagine 12 and the modified oligosaccharide to asparagine 60.
机构信息
Department of Biology C-016, University of California, San Diego, La Jolla, California 92093.
出版信息
Plant Physiol. 1986 May;81(1):320-2. doi: 10.1104/pp.81.1.320.
Abstract
Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, has a high mannose and a modified (fucosylated) oligosaccharide on each polypeptide. Fractionation by high performance liquid chromatography of tryptic digests of [(3)H]fucose or [(3)H]glucosamine labeled phytohemagglutinin, followed by amino acid sequencing of the isolated glycopeptides, shows that the high mannose oligosaccharide is attached to Asn(12) and the modified oligosaccharide to Asn(60) of the protein. In animal glycoproteins, high mannose chains are rarely found at the N-terminal side of complex chains.
摘要
植物血球凝集素，菜豆的凝集素，每个多肽上都有一个高甘露糖和一个修饰（岩藻糖基化）的寡糖。用[(3)H]岩藻糖或[(3)H]葡萄糖胺标记的植物血凝素的胰蛋白酶消化物进行高效液相色谱分级，然后对分离的糖肽进行氨基酸测序，表明高甘露糖寡糖连接到蛋白的 Asn(12)，而修饰的寡糖连接到 Asn(60)。在动物糖蛋白中，高甘露糖链很少出现在复杂链的 N 端侧。
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