利用单克隆抗体对不同植物物种硝酸还原酶的免疫学比较。
Immunological comparisons of nitrate reductase of different plant species using monoclonal antibodies.
机构信息
Laboratoire de Biologie Cellulaire, INRA, F 78000 Versailles, France.
出版信息
Plant Physiol. 1986 Jun;81(2):376-8. doi: 10.1104/pp.81.2.376.
Abstract
Six monoclonal antibodies against different epitopes of maize leaf nitrate reductase were used to compare plant nitrate reductases in enzyme linked immunosorbent assay and enzyme activity inhibition tests. The number of cross-reacting antibodies was shown to vary with species according to phylogenetic classification, ranging from five (sugarcane) to one (dicotyledonous species). Cross-reactions were restricted to higher plant nitrate reductases.
摘要
六种针对玉米叶片硝酸还原酶不同表位的单克隆抗体被用于酶联免疫吸附测定和酶活性抑制试验中，以比较植物硝酸还原酶。根据系统发生分类，交叉反应抗体的数量因物种而异，范围从 5 种（甘蔗）到 1 种（双子叶植物）。交叉反应仅限于高等植物硝酸还原酶。
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