单克隆抗体可识别玉米叶片硝酸还原酶上的多个表位。
Monoclonal antibodies identify multiple epitopes on maize leaf nitrate reductase.
作者信息
Cherel I, Grosclaude J, Rouze P
出版信息
Biochem Biophys Res Commun. 1985 Jun 28;129(3):686-93. doi: 10.1016/0006-291x(85)91946-1.
Abstract
Nine hybridoma cell lines secreting antibodies against the maize leaf nitrate reductase have been distinguished by reciprocal competition for binding to the antigenic site. Inhibition of enzymatic activities, and western blots of native enzyme and denatured subunits revealed different behaviors of individual antibodies towards the antigen. Two classes of monoclonal antibodies are inhibitory of NADH and methyl viologen nitrate reductase activities, but only one affects also NADH cytochrome c reductase activity. The associated epitopes are sensitive to antigen conformation. Among the 4 other classes, one is specific for the native conformation of the molecule, another binds more strongly to the denatured antigen, and two recognize equally well the two forms.
摘要
通过与抗原位点结合的相互竞争，已区分出九种分泌抗玉米叶片硝酸还原酶抗体的杂交瘤细胞系。酶活性抑制以及天然酶和变性亚基的蛋白质免疫印迹显示，各个抗体对抗原的行为不同。两类单克隆抗体可抑制NADH和甲基紫精硝酸还原酶活性，但只有一种也会影响NADH细胞色素c还原酶活性。相关表位对抗原构象敏感。在其他四类中，一类对分子的天然构象具有特异性，另一类与变性抗原结合更强，还有两类对两种形式的识别效果相同。
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