植物生长调节剂的单克隆抗体：III. 玉米素核苷和二氢玉米素核苷。
Monoclonal Antibodies to Plant Growth Regulators: III. Zeatinriboside and Dihydrozeatinriboside.
机构信息
Universität Osnabrück, Fachbereich Biologie/Chemie, Postfach 4469, D-4500 Osnabrück, West Germany.
出版信息
Plant Physiol. 1986 Jun;81(2):516-21. doi: 10.1104/pp.81.2.516.
Abstract
Four high affinity monoclonal antibodies, which recognize two plant growth regulators from the cytokinin group, namely trans-zeatin riboside and dihydrozeatin riboside and their derivatives are reported. Six hybridomas were produced from three independent fusions of Balb/c spleen cells with P3-NS1-Ag 4-1 (abbreviated NS1) or X63-Ag 8.653 (X63) myeloma cells. The mice had been hyperimmunized with zeatin riboside-bovine serum albumin conjugate or dihydrozeatin riboside-bovine serum albumin conjugate for 3 months. The hybridomas secrete antibodies of the IgG 1 or IgG 2b subclass and allow the detection of femtomole amounts of the free cytokinins, their ribosides, and ribotides in plant extracts. The use of these monoclonals in radio- and enzyme-linked immunosorbent assay is also discussed.
摘要
报道了 4 种高亲和力的单克隆抗体，它们可以识别两种来自细胞分裂素组的植物生长调节剂，即玉米素核苷和二氢玉米素核苷及其衍生物。从 3 次独立融合的 Balb/c 脾细胞与 P3-NS1-Ag 4-1（简称 NS1）或 X63-Ag 8.653（X63）骨髓瘤细胞中产生了 6 株杂交瘤。这些小鼠经过 3 个月的玉米素核苷-牛血清白蛋白缀合物或二氢玉米素核苷-牛血清白蛋白缀合物的超免疫接种。杂交瘤分泌 IgG1 或 IgG2b 亚类的抗体，允许在植物提取物中检测到皮摩尔数量的游离细胞分裂素、它们的核苷和核苷酸。还讨论了这些单克隆抗体在放射免疫和酶联免疫吸附测定中的应用。
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