Biologie I, Universität Tübingen, D-7400 Tübingen 1, Federal Republic of Germany.
Plant Physiol. 1986 Jul;81(3):854-8. doi: 10.1104/pp.81.3.854.
A semiquantitative assay for light-dependent O(2) evolution by a single mesophyll protoplast is described. The assay indicator is the density of aerotactic bacteria (Pseudomonas aeruginosa, ATCC 10145; ;Engelmann experiment') attracted to the protoplast. Quantification is by dark field microphotometry. The sensitivity is about 50 femtomoles O(2) per protoplast per minute. The results demonstrate the biphasic nature of O(2) evolution of a single protoplast during photosynthetic induction. Computerized data acquisition yields traces which, until a steady state of photosynthetic O(2) evolution is reached, are identical to ordinary O(2) electrode traces.
描述了一种用于测量单个叶肉原生质体光依赖性 O(2) 释放的半定量分析方法。该测定的指示物是被原生质体吸引的趋氧菌（铜绿假单胞菌，ATCC 10145；恩格尔曼实验）的密度。定量分析通过暗场显微光度计进行。灵敏度约为每分钟每个原生质体 50 飞摩尔 O(2)。结果表明，在光合作用诱导过程中，单个原生质体的 O(2)释放具有两相性。计算机化数据采集得到的轨迹在达到光合作用 O(2)释放的稳定状态之前与普通 O(2)电极轨迹相同。
