Mehrle W, Hampp R, Naton B, Grothe D
Universitat Tubingen, Abteilung Biochemie der Pflanzen, Federal Republic of Germany.
Plant Physiol. 1989;89(4):1172-7. doi: 10.1104/pp.89.4.1172.
Electrofusion of evacuolated with vacuolated mesophyll protoplasts of Nicotiana tabacum was performed as part of the German Sounding Rocket Program (TEXUS 17, 1988). The results indicate a significant increase not only in the yield of 1:1 hybrids, but also in homo- and multifusion products. Hybrids obtained under microgravity have been shown to be viable to a higher degree with respect to their ability for light-dependent O2-evolution (independent of other substrates than bicarbonate). This finding is of interest for fusion experiments where only limited numbers of fusion partners are available (e.g. protoplasts from embryogenic tissues) or where fusion yields are extremely low under 1 x gravity (e.g. protoplasts of different specific density).
作为德国探空火箭计划（TEXUS 17，1988年）的一部分，进行了烟草液泡化叶肉原生质体与液泡化叶肉原生质体的电融合。结果表明，不仅1:1杂种的产量显著增加，同核融合和多核融合产物的产量也显著增加。已证明在微重力条件下获得的杂种在依赖光的O2释放能力方面（除碳酸氢盐外不依赖其他底物）具有更高的活力。这一发现对于那些只有有限数量融合伙伴可用的融合实验（例如来自胚性组织的原生质体）或在1倍重力下融合产量极低的实验（例如不同比重的原生质体）来说是有意义的。
