能量促进玉米根中钠离子的摄取：通过 P 和 Na NMR 研究。
Energy Facilitated Na Uptake in Excised Corn Roots via P and Na NMR.
机构信息
United States Department of Agriculture, Agricultural Research Service, North Atlantic Area, Eastern Regional Research Center, Philadelphia, Pennsylvania 19118.
出版信息
Plant Physiol. 1986 Jul;81(3):925-8. doi: 10.1104/pp.81.3.925.
Abstract
The uptake of sodium ions by excised corn root tips (Zea mays L. cv FRB-73) was monitored by (23)Na nuclear magnetic resonance (NMR) methods in the presence of a membrane impermeable shift reagent under different metabolic conditions. In addition, for the first time, the energy status as well as the intracellular pH associated with this influx was concurrently evaluated by (31)P NMR. The rate of sodium ion uptake decreased (in order) as the normal metabolic state was changed by the addition of cyanide, anaerobic condition, and carbonyl cyanide trifluoromethoxy phenyl-hydrazone treatment. The results suggest that the proton electrochemical potential of the plasma membrane may facilitate the influx of Na(+).
摘要
采用（23）Na 核磁共振（NMR）方法，在存在膜不可渗透的位移试剂的情况下，监测离体玉米根尖（Zea mays L. cv FRB-73）对钠离子的摄取情况，并在不同代谢条件下进行。此外，本文首次通过（31）P NMR 同时评估了与这种内流相关的能量状态和细胞内 pH 值。钠离子摄取的速率（按顺序）随着正常代谢状态的变化而降低，具体变化方式为添加氰化物、无氧条件和三氟甲氧基苯腙羰基氰化物处理。结果表明，质膜的质子电化学势可能有利于 Na(+)的内流。
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