体内阳离子转运的直接高分辨率核磁共振研究，酵母细胞中的钠离子转运。
Direct high-resolution nuclear magnetic resonance studies of cation transport in vivo, Na+ transport in yeast cells.
作者信息
Balschi J A, Cirillo V P, Springer C S
出版信息
Biophys J. 1982 Jun;38(3):323-6. doi: 10.1016/S0006-3495(82)84566-9.
Abstract
A new nuclear magnetic resonance (NMR) method for monitoring transmembrane metal cation transport is reported. It is illustrated with a study of Na(+) efflux from Na(+)-rich yeast cells. The technique involves the use of an anionic paramagnetic shift reagent, present only outside the cells, to induce a splitting of the sodium-23 NMR peak, in this case, into components representing intra- and extracellular Na(+). The time course of the efflux is in good agreement with the literature and can be well fitted with a double exponential decay expression. Splitting of the lithium-7 NMR signal from a suspension of Li(+)-rich respiratory-deficient, petite yeasts is also reported.
摘要
报道了一种用于监测跨膜金属阳离子转运的新型核磁共振（NMR）方法。通过对富含Na⁺的酵母细胞中Na⁺外流的研究进行了说明。该技术涉及使用仅存在于细胞外的阴离子顺磁位移试剂，以诱导钠-23 NMR峰分裂，在这种情况下，分裂为代表细胞内和细胞外Na⁺的组分。外流的时间进程与文献报道吻合良好，并且可以用双指数衰减表达式很好地拟合。还报道了来自富含Li⁺的呼吸缺陷型小酵母悬浮液中锂-7 NMR信号的分裂。
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