豇豆根瘤线粒体的制备和性质。
Preparation and properties of mitochondria from cowpea nodules.
机构信息
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853.
出版信息
Plant Physiol. 1986 Aug;81(4):1092-6. doi: 10.1104/pp.81.4.1092.
Abstract
Mitochondria were isolated from nodules of cowpea (Vigna unguiculata (L). Walp.) and purified on a Percoll gradient. They were only slightly contaminated by bacteroids (an average of 3.5%), and had low lipoxygenase activity. Compared to mitochondria from hypocotyls the nodule mitochondria had similar O(2) uptake rates and respiratory control ratios. The ADP/O ratios for both preparations were 1.4 to 1.7 and 2.3 to 2.6 with succinate and malate, respectively. Whereas mitochondria isolated from etiolated cowpea hypocotyls had 14 to 18% of their respiration insensitive to KCN, the respiration of nodule mitochondria was completely inhibited by KCN. Enzyme activities of nodule mitochondria were similar to those found in hypocotyl mitochondria, except for NAD(+)-malic enzyme which was 12-fold lower in the mitochondria from nodules.
摘要
从豇豆（Vigna unguiculata (L). Walp.）根瘤中分离线粒体，并在聚蔗糖梯度上进行纯化。它们仅被类菌体轻微污染（平均为 3.5%），且脂氧合酶活性较低。与下胚轴线粒体相比，根瘤线粒体具有相似的 O(2)摄取率和呼吸控制比。用琥珀酸和苹果酸作为底物时，两种制剂的 ADP/O 比值分别为 1.4 至 1.7 和 2.3 至 2.6。而从黄化豇豆下胚轴中分离的线粒体，有 14%至 18%的呼吸对 KCN 不敏感，而根瘤线粒体的呼吸则完全被 KCN 抑制。根瘤线粒体的酶活性与下胚轴线粒体相似，除了 NAD(+)-苹果酸酶，它在根瘤线粒体中的活性低 12 倍。
相似文献
1
Preparation and properties of mitochondria from cowpea nodules.豇豆根瘤线粒体的制备和性质。
Plant Physiol. 1986 Aug;81(4):1092-6. doi: 10.1104/pp.81.4.1092.
2
Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.豆科植物根瘤线粒体的微氧条件下的代谢。
Plant Physiol. 1986 Aug;81(4):1097-102. doi: 10.1104/pp.81.4.1097.
3
Isolation of Mitochondria from Leaf Tissue of Panicum miliaceum, a NAD-Malic Enzyme Type C(4) Plant.从属于 NAD-Malic 酶类型 C(4)植物的小米叶片组织中分离线粒体。
Plant Physiol. 1983 Jan;71(1):24-9. doi: 10.1104/pp.71.1.24.
4
De Novo Purine Synthesis in Nitrogen-Fixing Nodules of Cowpea (Vigna unguiculata [L.] Walp.) and Soybean (Glycine max [L.] Merr.).根瘤固氮过程中豇豆（Vigna unguiculata [L.] Walp.）和大豆（Glycine max [L.] Merr.）中新嘌呤的从头合成。
Plant Physiol. 1982 Jul;70(1):55-60. doi: 10.1104/pp.70.1.55.
5
In vitro plant regeneration via organogenesis of cowpea [Vigna unguiculata (L.) Walp.].通过豇豆[Vigna unguiculata (L.) Walp.]器官发生进行的体外植株再生
Plant Cell Rep. 1997 Dec;17(2):89-95. doi: 10.1007/s002990050358.
6
Effect of pO(2) during Growth on the Gaseous Diffusional Properties of Nodules of Cowpea (Vigna unguiculata L. Walp.).生长过程中氧分压对豇豆（菜豆属）结瘤气体扩散特性的影响。
Plant Physiol. 1990 Jul;93(3):956-61. doi: 10.1104/pp.93.3.956.
7
Analysis of a ferric leghemoglobin reductase from cowpea (Vigna unguiculata) root nodules.豇豆（Vigna unguiculata）根瘤中铁血红蛋白还原酶的分析。
Plant Sci. 2000 May 29;154(2):161-170. doi: 10.1016/s0168-9452(99)00272-1.
8
Isoenzymes of Superoxide Dismutase in Nodules of Phaseolus vulgaris L., Pisum sativum L., and Vigna unguiculata (L.) Walp.菜豆、豌豆和豇豆结瘤中超氧化物歧化酶同工酶的研究
Plant Physiol. 1989 Aug;90(4):1286-92. doi: 10.1104/pp.90.4.1286.
9
Cyanide-insensitive and Cyanide-sensitive O(2) Uptake in Wheat: II. GRADIENT-PURIFIED MITOCHONDRIA LACK CYANIDE-INSENSITIVE RESPIRATION.氰化物不敏感和敏感的 O(2) 摄取在小麦中：Ⅱ. 梯度纯化的线粒体缺乏氰化物不敏感的呼吸。
Plant Physiol. 1981 Mar;67(3):594-6. doi: 10.1104/pp.67.3.594.
10
Malate Oxidation and Cyanide-Insensitive Respiration in Avocado Mitochondria during the Climacteric Cycle.在鳄梨线粒体的呼吸作用中，苹果酸的氧化作用和对氰化物的不敏感性与呼吸作用的周期性变化有关。
Plant Physiol. 1982 Nov;70(5):1385-90. doi: 10.1104/pp.70.5.1385.
引用本文的文献
1
Malate Transport and Metabolism in Nitrogen-Fixing Legume Nodules.氮固定豆科植物根瘤中的苹果酸转运和代谢。
Molecules. 2021 Nov 15;26(22):6876. doi: 10.3390/molecules26226876.
2
Genome-scale metabolic reconstruction of the symbiosis between a leguminous plant and a nitrogen-fixing bacterium.豆科植物与固氮菌共生的基因组规模代谢重建。
Nat Commun. 2020 May 22;11(1):2574. doi: 10.1038/s41467-020-16484-2.
3
Activation of O(2) Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid.顺式、反式脱落酸对蓖麻籽叶线粒体中氧摄取及NAD特异性异柠檬酸脱氢酶的激活作用
Plant Physiol. 1990 Jan;92(1):147-50. doi: 10.1104/pp.92.1.147.
4
Nucleoside diphosphatase and 5'-nucleotidase activities of soybean root nodules and other tissues.大豆根瘤和其他组织的核苷二磷酸酶和 5'-核苷酸酶活性。
Plant Physiol. 1988 May;87(1):36-40. doi: 10.1104/pp.87.1.36.
5
Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.豆科植物根瘤线粒体的微氧条件下的代谢。
Plant Physiol. 1986 Aug;81(4):1097-102. doi: 10.1104/pp.81.4.1097.
6
A Re-Evaluation of the Role of the Infected Cell in the Control of O2 Diffusion in Legume Nodules.对受感染细胞在豆科植物根瘤中氧气扩散控制作用的重新评估
Plant Physiol. 1994 Aug;105(4):1321-1333. doi: 10.1104/pp.105.4.1321.
7
The site of oxygen limitation in soybean nodules.大豆根瘤中氧气限制的部位。
Plant Physiol. 1999 Feb;119(2):399-408. doi: 10.1104/pp.119.2.399.
本文引用的文献
1
Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.豆科植物根瘤线粒体的微氧条件下的代谢。
Plant Physiol. 1986 Aug;81(4):1097-102. doi: 10.1104/pp.81.4.1097.
2
Alternative pathway respiration and lipoxygenase activity in aged potato slice mitochondria.衰老马铃薯切片线粒体中的替代途径呼吸和脂氧合酶活性。
Plant Physiol. 1982 Jun;69(6):1435-8. doi: 10.1104/pp.69.6.1435.
3
Organic Acid contents of soybean: age and source of nitrogen.大豆的有机酸含量：氮的年龄和来源。
Plant Physiol. 1981 Nov;68(5):989-91. doi: 10.1104/pp.68.5.989.
4
Disulfiram inhibition of the alternative respiratory pathway in plant mitochondria.二硫化碳抑制植物线粒体的替代呼吸途径。
Plant Physiol. 1981 Aug;68(2):393-400. doi: 10.1104/pp.68.2.393.
5
Effect of NAD on Malate Oxidation in Intact Plant Mitochondria.NAD 对完整植物线粒体中苹果酸氧化的影响。
Plant Physiol. 1980 Aug;66(2):225-9. doi: 10.1104/pp.66.2.225.
6
Isolation of bacteria, transforming bacteria, and bacteroids from soybean nodules.从大豆根瘤中分离细菌、转化细菌和类菌体。
Plant Physiol. 1977 Nov;60(5):771-4. doi: 10.1104/pp.60.5.771.
7
Regulation of Succinate Dehyrogenase in Higher Plants: I. Some General Characteristics of the Membrane-bound Enzyme.高等植物琥珀酸脱氢酶的调节：I. 膜结合酶的一些一般特征。
Plant Physiol. 1973 Dec;52(6):616-21. doi: 10.1104/pp.52.6.616.
8
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
9
Effect of bicarbonate and oxaloacetate on malate oxidation by spinach leaf mitochondria.碳酸氢盐和草酰乙酸对菠菜叶片线粒体苹果酸氧化的影响。
Biochim Biophys Acta. 1980 Feb 8;589(2):176-89. doi: 10.1016/0005-2728(80)90036-5.
10
Regulation of malate oxidation in plant mitochondria. Response to rotenone and exogenous NAD+.植物线粒体中苹果酸氧化的调节。对鱼藤酮和外源NAD⁺的反应。
Biochem J. 1982 Dec 15;208(3):703-11. doi: 10.1042/bj2080703.
Zotero 插件