豆科植物根瘤线粒体的微氧条件下的代谢。
Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.
机构信息
Boyce Thompson Institute, Tower Road, Ithaca, New York 14853.
出版信息
Plant Physiol. 1986 Aug;81(4):1097-102. doi: 10.1104/pp.81.4.1097.
Abstract
A method is described for isolating mitochondria from nodules of cowpea (Vigna unguiculata [L.] Walp.) under completely anaerobic conditions. The mitochondria were immediately active when incubated aerobically with substrates, and their respiration rates were higher than mitochondria prepared in air. The mitochondria lacked fumarate reductase and were not inhibited by 5% CO(2). When incubated under microaerobic conditions, their respiration could be measured by leghemoglobin spectroscopy. Microaerobic respiration was inhibited approximately 50% by 1 millimolar malonate, and was completely inhibited by cyanide. O(2) uptake and the ATP/O ratio declined under microaerobic conditions, and therefore ATP production may be low in the environment of infected nodule cells.
摘要
介绍了一种在完全厌氧条件下从豇豆（Vigna unguiculata [L.] Walp.）根瘤中分离线粒体的方法。在有氧条件下与底物孵育时，线粒体立即变得活跃，其呼吸速率高于在空气中制备的线粒体。这些线粒体缺乏延胡索酸还原酶，并且不受 5% CO(2)的抑制。在微氧条件下孵育时，可以通过豆血红蛋白光谱法测量其呼吸作用。1 毫摩尔丙二酸盐可抑制微氧呼吸约 50%，氰化物可完全抑制微氧呼吸。微氧条件下 O(2)摄取和 ATP/O 比值下降，因此感染的根瘤细胞环境中 ATP 生成可能较低。
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