Department of Biology, Virginia Tech, Blacksburg, Virginia 24061.
Plant Physiol. 1986 Dec;82(4):1161-3. doi: 10.1104/pp.82.4.1161.
The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase was measured in extracts of leaves collected before dawn (predawn activity, pa) and at midday (midday activity, ma). Twenty-three of the 37 species examined showed a pa/ma ratio (</=0.75, while only Capsicum frutescens, Cucumis sativa, Glycine max, Nicotiana tabacum, Vigna unguiculata, and 3 Solanum species showed a pa/ma ratio </=0.5. Phaseolus vulgaris consistently showed a pa/ma ratio of </=0.1. Activities and pa/ma ratios of the same species grown in the United States and the United Kingdom were very similar. Gel filtration of extracts before assay had no effect on the observed activities and the pa/ma ratios. These data are consistent with the hypothesis that in a number of species the enzyme is partially inhibited following the night period by the presence of a tight-binding inhibitor.
在黎明前(黎明前活性,pa)和中午(中午活性,ma)采集的叶片提取物中测量了核酮糖-1,5-二磷酸羧化酶/加氧酶的活性。在检查的 37 种物种中,有 23 种表现出 pa/ma 比值(</=0.75,而只有辣椒、黄瓜、大豆、烟草、豇豆和 3 种茄属植物以及番茄表现出 pa/ma 比值 </=0.5。菜豆始终表现出 pa/ma 比值 </=0.1。在美国和英国生长的相同物种的活性和 pa/ma 比值非常相似。在测定之前对提取物进行凝胶过滤对观察到的活性和 pa/ma 比值没有影响。这些数据与以下假设一致,即在许多物种中,酶在夜间会被一种紧密结合的抑制剂部分抑制。