Plant Cell Biology Research Center, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia.
Plant Physiol. 1986 Dec;82(4):885-9. doi: 10.1104/pp.82.4.885.
Arabinogalactan-proteins (AGPs), isolated from the pistils of Nicotiana alata, an ornamental tobacco, are developmentally regulated. Both the total amount and concentration of AGP in the stigma increase during flower development, reaching 10 micrograms AGP/stigma at maturity. In contrast, AGP concentration in the style remains constant throughout the maturation period reaching 12 micrograms AGP/style at maturity. The classes of AGP present in the stigma and style during flower development, separated according to their charge by crossed-electrophoresis, are different and change during development. Pollination of flowers of N. alata with compatible or incompatible pollen results in a significant and reproducible increase in the amount of AGPs in the stigma, but not the style, compared with control unpollinated pistils. Pollination with ethanol vapor inactivated pollen also results in an increase in the amount of AGP in the stigma, but this is less than half that observed following pollination with viable pollen. There are no significant differences in the classes of AGP, based on crossed-electrophoresis, present in the pistil following pollination.
阿拉伯半乳聚糖蛋白（AGP），从观赏烟草烟草柱花草中分离出来，其含量受发育调控。在花朵发育过程中，柱头中 AGP 的总量和浓度均增加，在成熟时达到 10 微克 AGP/柱头。相比之下，花柱中 AGP 的浓度在整个成熟期间保持不变，在成熟时达到 12 微克 AGP/花柱。根据交叉电泳的电荷分离，在花发育过程中柱头和花柱中存在的 AGP 类是不同的，并在发育过程中发生变化。与具有亲和性或不亲和性花粉的烟草柱花草花朵授粉，与对照未授粉的柱头相比，显著且可重复地增加柱头中 AGP 的含量，但花柱中 AGP 的含量没有变化。用乙醇蒸气使花粉失活进行授粉也会导致柱头中 AGP 含量增加，但与有活力花粉授粉相比，这一增加不到一半。授粉后，根据交叉电泳，柱心中存在的 AGP 类没有显著差异。
