荧光标记对融合性胡萝卜原生质体钙诱导融合的影响。

The effect of fluorescent labeling on calcium-induced fusion of fusogenic carrot protoplasts.

机构信息

Department of Botany, North Carolina State University, 27695-7612, NC, USA.

出版信息

Plant Cell Rep. 1986 Aug;5(4):252-5. doi: 10.1007/BF00269814.

Abstract

Various fluorescent compounds - carboxyfluorescein, scopoletin, fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), rhodamine 123, and rhodamine B ethyl ester - were used to study their effects on calcium-induced fusion of fusogenic carrot (Daucus carota L.) protoplasts. These protoplasts normally fused at a high percentage (50-60%) in response to 10 mM calcium, pH 6.0; however, if cells had been labeled with scopoletin, FITC, or RITC, fusion was greatly reduced. In contrast, labeling with carboxyfluorescein, rhodamine 123, or rhodamine B ethyl ester had no detectable effect on calcium-induced fusion. The two rhodamine dyes are shown to be localized in mitochondria.

摘要

各种荧光化合物 - 羧基荧光素、香豆素、异硫氰酸荧光素（FITC）、罗丹明 B 异硫氰酸酯（RITC）、罗丹明 123 和罗丹明 B 乙酯 - 被用于研究它们对钙离子诱导融合的融合性胡萝卜（Daucus carota L.）原生质体的影响。这些原生质体通常在响应 10 mM 钙离子、pH 值 6.0 时以高百分比（50-60%）融合；然而，如果细胞用香豆素、FITC 或 RITC 标记，则融合大大减少。相比之下，用羧基荧光素、罗丹明 123 或罗丹明 B 乙酯标记对钙离子诱导的融合没有明显影响。这两种罗丹明染料被证明定位于线粒体中。