• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过氢交换电喷雾电离质谱法探测蛋白质的构象变化。

Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry.

作者信息

Katta V, Chait B T

机构信息

Rockefeller University, New York, NY 10021.

出版信息

Rapid Commun Mass Spectrom. 1991 Apr;5(4):214-7. doi: 10.1002/rcm.1290050415.

DOI:10.1002/rcm.1290050415
PMID:1666528
Abstract

Hydrogen-exchange electrospray-ionization mass spectrometry is demonstrated to be an effective new method for probing conformational changes of proteins in solutions. The method is based on the mass spectrometric measurement of the extent of hydrogen/deuterium exchange that occurs in different protein conformers over defined periods of time. Results are presented in which hydrogen-exchange electrospray-ionization mass spectrometry is used to probe conformational changes in bovine ubiquitin induced by the addition of methanol to aqueous acidic solutions of the protein.

摘要

氢交换电喷雾电离质谱法被证明是探测溶液中蛋白质构象变化的一种有效的新方法。该方法基于对在规定时间段内不同蛋白质构象体中发生的氢/氘交换程度的质谱测量。本文给出了相关结果，其中氢交换电喷雾电离质谱法被用于探测在蛋白质的酸性水溶液中添加甲醇后牛泛素的构象变化。

相似文献

1
Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry.通过氢交换电喷雾电离质谱法探测蛋白质的构象变化。
Rapid Commun Mass Spectrom. 1991 Apr;5(4):214-7. doi: 10.1002/rcm.1290050415.
2
Hydrogen/deuterium exchange mass spectrometry.氢/氘交换质谱分析法
Methods Mol Biol. 2009;492:255-71. doi: 10.1007/978-1-59745-493-3_15.
3
Solvent-induced conformational changes of polypeptides probed by electrospray-ionization mass spectrometry.用电喷雾电离质谱法探测溶剂诱导的多肽构象变化
Rapid Commun Mass Spectrom. 1991 Mar;5(3):101-5. doi: 10.1002/rcm.1290050303.
4
Conformational heterogeneity of stability of apomyoglobin studied by hydrogen/deuterium exchange and electrospray ionization mass spectrometry.通过氢/氘交换和电喷雾电离质谱法研究脱辅基肌红蛋白稳定性的构象异质性。
Biochemistry. 1996 Apr 2;35(13):4069-78. doi: 10.1021/bi9521304.
5
Conformational changes in beta-endorphin as studied by electrospray ionization mass spectrometry.通过电喷雾电离质谱法研究β-内啡肽的构象变化。
Rapid Commun Mass Spectrom. 2001;15(23):2341-6. doi: 10.1002/rcm.513.
6
Using mass spectrometry to probe the subtle differences in conformations of several cytochromes c in aqueous and methanol solutions.
J Mass Spectrom. 2004 Dec;39(12):1523-30. doi: 10.1002/jms.757.
7
Hydrogen exchange mass spectrometry for the analysis of protein dynamics.用于蛋白质动力学分析的氢交换质谱法。
Mass Spectrom Rev. 2006 Jan-Feb;25(1):158-70. doi: 10.1002/mas.20064.
8
Thermal denaturation of Escherichia coli thioredoxin studied by hydrogen/deuterium exchange and electrospray ionization mass spectrometry: monitoring a two-state protein unfolding transition.通过氢/氘交换和电喷雾电离质谱法研究大肠杆菌硫氧还蛋白的热变性：监测两态蛋白质的解折叠转变
Biochemistry. 1999 Jan 19;38(3):1136-43. doi: 10.1021/bi981938w.
9
Hydrogen exchange properties of proteins in native and denatured states monitored by mass spectrometry and NMR.通过质谱和核磁共振监测天然和变性状态下蛋白质的氢交换特性。
Protein Sci. 1997 Jun;6(6):1316-24. doi: 10.1002/pro.5560060620.
10
Probing backbone dynamics with hydrogen/deuterium exchange mass spectrometry.利用氢/氘交换质谱法探究主链动力学
Methods Mol Biol. 2014;1084:81-99. doi: 10.1007/978-1-62703-658-0_5.

引用本文的文献

1
Kingfisher: An open-sourced web-based platform for the analysis of hydrogen exchange mass spectrometry data.翠鸟：一个用于分析氢交换质谱数据的基于网络的开源平台。
Protein Sci. 2025 Apr;34(4):e70096. doi: 10.1002/pro.70096.
2
Hydrogen/Deuterium Exchange Mass Spectrometry: Fundamentals, Limitations, and Opportunities.氢/氘交换质谱法：基础、局限性与机遇
Mol Cell Proteomics. 2024 Nov;23(11):100853. doi: 10.1016/j.mcpro.2024.100853. Epub 2024 Oct 9.
3
Examining DNA Structures with In-droplet Hydrogen/Deuterium Exchange Mass Spectrometry.
利用液滴内氢/氘交换质谱法检测DNA结构
Int J Mass Spectrom. 2024 May;499. doi: 10.1016/j.ijms.2024.117231. Epub 2024 Mar 7.
4
Hydrogen Deuterium Exchange and other Mass Spectrometry-based Approaches for Epitope Mapping.用于表位作图的氢氘交换及其他基于质谱的方法。
Front Anal Sci. 2023;3. doi: 10.3389/frans.2023.1118749. Epub 2023 May 18.
5
In-droplet hydrogen-deuterium exchange to examine protein/peptide solution conformer heterogeneity.液滴内氢氘交换实验研究蛋白质/肽溶液构象异质性。
Rapid Commun Mass Spectrom. 2023 Aug 30;37(16):e9593. doi: 10.1002/rcm.9593.
6
Zero-Degree Celsius Capillary Electrophoresis Electrospray Ionization for Hydrogen Exchange Mass Spectrometry.零摄氏度毛细管电泳电喷雾电离用于氢交换质谱法。
Anal Chem. 2023 Jan 17;95(2):1149-1158. doi: 10.1021/acs.analchem.2c03893. Epub 2022 Dec 22.
7
Capillary Vibrating Sharp-Edge Spray Ionization Augments Field-Free Ionization Techniques to Promote Conformer Preservation in the Gas-Phase for Intractable Biomolecular Ions.毛细管振动锐边喷雾电离增强无场电离技术，以促进难处理生物分子离子在气相中的构象保持。
J Phys Chem B. 2022 Nov 10;126(44):8970-8984. doi: 10.1021/acs.jpcb.2c04960. Epub 2022 Nov 1.
8
Using hydrogen-deuterium exchange mass spectrometry to characterize Mtr4 interactions with RNA.利用氢氘交换质谱技术来鉴定 Mtr4 与 RNA 的相互作用。
Methods Enzymol. 2022;673:475-516. doi: 10.1016/bs.mie.2022.04.002. Epub 2022 May 14.
9
Top-Down Detection of Oxidative Protein Footprinting by Collision-Induced Dissociation, Electron-Transfer Dissociation, and Electron-Capture Dissociation.自上而下通过碰撞诱导解离、电子转移解离和电子俘获解离检测氧化蛋白质足迹。
Anal Chem. 2022 Jul 19;94(28):9993-10002. doi: 10.1021/acs.analchem.1c05476. Epub 2022 Jul 7.
10
HDfleX: Software for Flexible High Structural Resolution of Hydrogen/Deuterium-Exchange Mass Spectrometry Data.HDfleX：用于灵活的高结构分辨率氢/氘交换质谱数据的软件。
Anal Chem. 2022 Mar 22;94(11):4557-4564. doi: 10.1021/acs.analchem.1c05339. Epub 2022 Mar 9.