CIBA-GEIGY Biotechnology Unit, P. O. Box 12257, Research Triangle Park, North Carolina 27709.
Plant Physiol. 1987 Jun;84(2):438-42. doi: 10.1104/pp.84.2.438.
Peroxidases (EC 1.11.1.7) have been implicated in the responses of plants to physical stress and to pathogens, as well as in a variety of cellular processes including cell wall biosynthesis. Tissue samples from leaf, root, pith, and callus of Nicotiana tabacum were assayed for specific peroxidase isozymes by analytical isoelectric focusing. Each tissue type was found to exhibit a unique isozyme fingerprint. Root tissue expressed all of the detectable peroxidase isozymes in the tobacco plant, whereas each of the other tissues examined expressed a different subset of these isozymes. In an effort to determine which peroxidase isozymes from Nicotiana tabacum are involved in cell wall biosynthesis or other normal cellular functions and which respond to stress, plants were subjected to either wounding or infection with tobacco mosaic virus. Wounding the plant triggered the expression of several cationic isozymes in the leaf and both cationic and anionic isozymes in pith tissue. Maximum enzyme activity was detected at 72 hours after wounding, and cycloheximide treatment prevented this induction. Infection of tobacco with tobacco mosaic virus induced two moderately anionic isozymes in the leaves in which virus was applied and also systemically induced in leaves which were not inoculated with virus.
过氧化物酶（EC 1.11.1.7）被认为与植物对物理胁迫和病原体的反应以及各种细胞过程有关，包括细胞壁生物合成。通过分析等电聚焦，对来自烟草叶片、根、髓和愈伤组织的组织样本进行了特定过氧化物酶同工酶的测定。发现每种组织类型都表现出独特的同工酶指纹。根组织表达了烟草中所有可检测到的过氧化物酶同工酶，而检查的其他每种组织都表达了这些同工酶的不同子集。为了确定参与细胞壁生物合成或其他正常细胞功能的烟草过氧化物酶同工酶以及对胁迫有反应的同工酶，对植物进行了创伤或感染烟草花叶病毒处理。创伤植物触发了叶片中几种阳离子同工酶的表达，以及髓组织中阳离子和阴离子同工酶的表达。在创伤后 72 小时检测到最大酶活性，且环己酰亚胺处理可阻止这种诱导。烟草感染烟草花叶病毒在接种病毒的叶片中诱导了两种中等阴离子同工酶，并且在未接种病毒的叶片中也系统地诱导了这些同工酶。
