Department of Biotechnology, Faculty of Engineering, Osaka University, Suita, 565, Osaka, Japan.
Plant Cell Rep. 1994 Jan;13(3-4):149-54. doi: 10.1007/BF00239882.
Peroxidases have been implicated in the responses of plants to physiological stress and to pathogens. Wound-induced peroxidase of horseradish (Armoracia rusticana) was studied. Total peroxidase activity was increased by wounding in cell wall fractions extracted from roots, stems and leaves of horseradish. On the other hand, wounding decreased the peroxidase activity in the soluble fraction from roots. The enzyme activities of the basic isozymes were induced by wounding in horseradish leaves based on data obtained by fractionation of crude enzyme in isoelectric focusing gel electrophoresis followed by activity staining. We have previously isolated genomic clones for four peroxidase genes, namely, prxC1a, prxC1b, prxC2 and prxC3. Northern blot analysis using gene-specific probes showed that mRNA of prxC2, which encodes a basic isozyme, accumulated by wounding, while the mRNAs for other peroxidase genes were not induced. Tobacco (Nicotiana tabacum) plants were transformed with four chimeric gene constructs, each consisting of a promoter from one of the peroxidase genes and the β-glucuronidase (GUS) structural gene. High level GUS activity induced in response to wounding was observed in tobacco plants containing the prxC2-GUS construct.
过氧化物酶参与了植物对生理胁迫和病原体的反应。研究了辣根（Armoracia rusticana）的诱导型过氧化物酶。在从辣根的根、茎和叶中提取的细胞壁部分中，总过氧化物酶活性因受伤而增加。另一方面，受伤会降低根中可溶性部分的过氧化物酶活性。根据在等电聚焦凝胶电泳中对粗酶进行分级分离并随后进行活性染色获得的数据，基本同工酶的酶活性在辣根叶片中因受伤而被诱导。我们之前已经分离了四个过氧化物酶基因的基因组克隆，即 prxC1a、prxC1b、prxC2 和 prxC3。使用基因特异性探针的 Northern blot 分析表明，编码碱性同工酶的 prxC2 的 mRNA 因受伤而积累，而其他过氧化物酶基因的 mRNA 未被诱导。烟草（Nicotiana tabacum）植物用四个嵌合基因构建体转化，每个构建体由一个过氧化物酶基因的启动子和β-葡萄糖醛酸酶（GUS）结构基因组成。在含有 prxC2-GUS 构建体的烟草植物中观察到对受伤的高水平 GUS 活性诱导。
