高粱叶片磷酸烯醇式丙酮酸羧化酶的光调控：信使 RNA 和光受体的特性。

Laboratoire de Physiologie Végétale Moléculaire, CNRS (UA No. 1128), Université de Paris-Sud, Orsay, France.

Plant Physiol. 1987 Sep;85(1):243-6. doi: 10.1104/pp.85.1.243.

The mechanism underlying the light effect on phosphoenolpyruvate carboxylase (PEPC) from the C(4) plant sorghum (Sorghum vulgare Pers., var Tamaran) leaves was investigated. Following exposure to light a new isozyme of PEPC, specific for the green leaf and responsible for primary CO(2) fixation in photosynthesis, was established. Northern blot experiments revealed the presence of PEPC mRNA showing a molecular weight of 3.4 kilobases. During the greening process, concomitant to enzyme activity, PEPC protein and PEPC messenger RNA amounts increased considerably. This photoresponse was shown to be under phytochrome control.

我们研究了高粱（ Sorghum vulgare Pers.，var Tamaran）叶片 C4 植物草酰乙酸磷酸化酶（PEPC）光效应的作用机制。在光照后，新的 PEPC 同工酶被建立，该同工酶专用于绿色叶片，负责光合作用中的初级 CO2 固定。Northern blot 实验揭示了存在分子量为 3.4 千碱基的 PEPC mRNA。在绿色化过程中，伴随着酶活性，PEPC 蛋白和 PEPC 信使 RNA 含量显著增加。这种光反应受光敏色素的控制。