Hermans J, Westhoff P
Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine Universität Düsseldorf, Federal Republic of Germany.
Mol Gen Genet. 1990 Dec;224(3):459-68. doi: 10.1007/BF00262441.
Phosphoenolpyruvate carboxylase (PEPCase) was shown to be encoded by a multigene family in various Flaveria species analysed. Several clones were isolated from genomic libraries of F. pringlei (C3 species) and F. trinervia (C4 species) and classified into four distinct groups according to their hybridization behaviour to a full-length cDNA clone encoding the PEPCase C4 isozyme of F. trinervia. A detailed cross-hybridization analysis demonstrated that the closest relative of most of the PEPCase genes isolated from F. trinervia and F. pringlei was not found in the same but in the other species. Northern analysis, using stringent conditions, allowed discrimination of class-specific PEPCase transcripts and revealed characteristic organ-specific expression patterns.
在已分析的多种黄顶菊属植物中，磷酸烯醇式丙酮酸羧化酶（PEPCase）由一个多基因家族编码。从普氏黄顶菊（C3植物）和三脉黄顶菊（C4植物）的基因组文库中分离出了几个克隆，并根据它们与编码三脉黄顶菊PEPCase C4同工酶的全长cDNA克隆的杂交行为，将其分为四个不同的组。详细的交叉杂交分析表明，从三脉黄顶菊和普氏黄顶菊中分离出的大多数PEPCase基因的最密切相关基因，并非存在于同一物种中，而是存在于另一个物种中。使用严格条件进行的Northern分析，能够区分特定类别的PEPCase转录本，并揭示出特征性的器官特异性表达模式。
