Nelson T, Harpster M H, Mayfield S P, Taylor W C
J Cell Biol. 1984 Feb;98(2):558-64. doi: 10.1083/jcb.98.2.558.
We have established schedules of expression during maize leaf development in light and darkness for the messenger RNAs (mRNAs) and polypeptides for ribulose 1,5-bisphosphate carboxylase (RuBPCase) subunits, phosphoenolpyruvate carboxylase (PEPCase), and the light-harvesting chlorophyll a/b-binding protein (LHCP). Levels of mRNAs were measured by hybridization with cloned probes, and proteins were measured by immunodetection on protein gel blots. The initial synthesis in leaves of all four mRNAs follows a light-independent schedule; illumination influences only the level to which each mRNA accumulates. The synthesis of RuBPCase small and large subunits and of PEPCase polypeptides also follows a light-independent schedule which is modified quantitatively by light. However, the accumulation of LHCP polypeptides absolutely requires illumination. The accumulation of each protein closely follows the accumulation of its mRNA during growth in light. Higher ratios of PEPCase and RuBPCase protein to mRNA occur during dark growth.
我们已经确定了在玉米叶片发育过程中，光照和黑暗条件下，1,5-二磷酸核酮糖羧化酶（RuBPCase）亚基、磷酸烯醇式丙酮酸羧化酶（PEPCase）以及光捕获叶绿素a/b结合蛋白（LHCP）的信使核糖核酸（mRNA）和多肽的表达时间表。通过与克隆探针杂交来测量mRNA水平，通过蛋白质凝胶印迹上的免疫检测来测量蛋白质水平。所有这四种mRNA在叶片中的初始合成遵循不依赖光照的时间表；光照仅影响每种mRNA积累的水平。RuBPCase小亚基和大亚基以及PEPCase多肽的合成也遵循不依赖光照的时间表，光照会对其进行定量修饰。然而，LHCP多肽的积累绝对需要光照。在光照下生长期间，每种蛋白质的积累与其mRNA的积累密切相关。在黑暗生长期间，PEPCase和RuBPCase蛋白与mRNA的比例更高。
