GC-SIM-MS 分析前吲哚-3-乙酸的快速简单纯化方法。
A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis.
机构信息
Department of Botany, University of Maryland, College Park, Maryland 20742.
出版信息
Plant Physiol. 1988 Mar;86(3):822-5. doi: 10.1104/pp.86.3.822.
Abstract
A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.
摘要
建立了一种使用氨基阴离子交换微柱和短高效 C18 柱在进行气相色谱-选择离子监测-质谱分析前对吲哚-3-乙酸进行纯化的简单快速方法。由于可以同时制备多个样品，因此该方法更快，样品制备时间减少到正常所需时间的三分之一。此外，与溶剂分配法相比，最终回收率提高了 40%至 50%。
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