菊芋块茎原生质体中的细胞质蔗糖合酶（Helianthus tuberosus L.）
Sucrose Synthase, a Cytosolic Enzyme in Protoplasts of Jerusalem Artichoke Tubers (Helianthus tuberosus L.).
机构信息
University of Zürich, Institute of Plant Biology, Zollikerstrasse 107, CH-8008 Zürich, Switzerland.
出版信息
Plant Physiol. 1988 Oct;88(2):239-41. doi: 10.1104/pp.88.2.239.
The exact subcellular location of sucrose synthase (UDP-d-glucose: d-fructose 2-alpha-d-glucosyltransferase, EC 2.4.1.13) in Helianthus tuberosus tubers was studied by comparison of its activity in protoplasts with that of vacuoles isolated from them. Assuming 100% of the beta-N-acetylglucosaminidase activity to be of vacuolar origin, less than 5% of both the sucrose synthase activity and the extravacuolar marker NAD-malate dehydrogenase was detected in the vacuole preparations. Sucrose synthase is therefore an extravacuolar enzyme. Its role in the inulin metabolism of H. tuberosus is discussed.
通过比较块茎原生质体和其中分离的液泡的蔗糖合酶（UDP-D-葡萄糖：D-果糖 2-α-D-葡萄糖基转移酶，EC2.4.1.13）活性，研究了其在Helianthus tuberosus 块茎中的确切亚细胞位置。假设β-N-乙酰氨基葡萄糖苷酶活性的 100％均来自液泡，在液泡制剂中仅检测到不到 5％的蔗糖合酶活性和细胞外标记 NAD-苹果酸脱氢酶。因此，蔗糖合酶是一种细胞外酶。讨论了它在 H. tuberosus 菊粉代谢中的作用。
Zotero 插件