基于高效液相色谱法对用 UDP-葡萄糖孵育甘蔗液泡产生的二糖的重新评价。
High Performance Liquid Chromatography-Based Reevaluation of Disaccharides Produced upon Incubation of Sugarcane Vacuoles with UDP-Glucose.
机构信息
Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701.
出版信息
Plant Physiol. 1988 Oct;88(2):266-9. doi: 10.1104/pp.88.2.266.
Abstract
A reanalysis of products formed after short-term incubation of sugarcane (Saccharum spp. hybrid cv H50-7209) vacuole preparations with uridine diphosphate [(14)C]glucose was performed. The results indicated that the ethanol-soluble substance previously identified as sucrose did not elute with sucrose when subjected to high performance liquid chromatography but had the same retention time as a disaccharide tentatively identified as laminaribiose.
摘要
对甘蔗（Saccharum spp. hybrid cv H50-7209）液泡制剂与尿苷二磷酸[14C]葡萄糖短期孵育后形成的产物进行了再分析。结果表明，以前鉴定为蔗糖的乙醇溶性物质在高效液相色谱中不与蔗糖一起洗脱，但与暂定鉴定为纤维二糖的二糖具有相同的保留时间。
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本文引用的文献
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Analysis of the reaction products from incubation of sugarcane vacuoles with uridine-diphosphate-glucose: no evidence for the group translocator.甘蔗液泡与尿苷二磷酸葡萄糖孵育反应产物的分析：无基团转运体的证据
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2
Vacuoles from Sugarcane Suspension Cultures : I. ISOLATION AND PARTIAL CHARACTERIZATION.甘蔗悬浮培养细胞的液泡：I. 分离和部分特性。
Plant Physiol. 1982 Jun;69(6):1315-9. doi: 10.1104/pp.69.6.1315.
3
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