Haynie S L, Whitesides G M
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
Appl Biochem Biotechnol. 1990 Feb;23(2):155-70. doi: 10.1007/BF02798384.
This paper describes cell-free enzymatic syntheses of sucrose and trehalose using partially-purified preparations of sucrose and trehalose synthetase. The coupling of the regeneration of uridine-5'-diphosphoglucose (UDP-Glc) with synthesis of the disaccharide offers a practical route to millimol quantities of these carbohydrates. The syntheses used pyruvate kinase, UDP-Glc pyrophosphorylase, and inorganic pyrophosphatase, and the regenerated UDP-Glc was cycled approximately 10 times.
本文描述了使用蔗糖和海藻糖合成酶的部分纯化制剂进行蔗糖和海藻糖的无细胞酶促合成。尿苷-5'-二磷酸葡萄糖（UDP-Glc）的再生与二糖合成的偶联为制备毫摩尔量的这些碳水化合物提供了一条实用途径。该合成使用了丙酮酸激酶、UDP-Glc焦磷酸化酶和无机焦磷酸酶，再生的UDP-Glc循环了约10次。
