Department of Biology, University of California, Los Angeles, California 90024.
Plant Physiol. 1989 Feb;89(2):602-9. doi: 10.1104/pp.89.2.602.
Distribution of the major light-harvesting chlorophyll a/b-protein (LHCII) and its mRNA within bundle sheath and mesophyll cells of maize (Zea mays L.) was studied using in situ immunolocalization and hybridization, respectively. In situ hybridization with specific LHCII RNA probes from maize and Lemna gibba definitively shows the presence of high levels of mRNA for LHCII in both bundle sheath cells and mesophyll cells. In situ immuno-localization studies, using an LHCII monoclonal antibody, demonstrate the presence of LHCII polypeptides in chloroplasts of both cell types. The polypeptide composition of LHCII and the amount of LHCII in bundle sheath cells are different from those in mesophyll cells. Both mesophyll and bundle sheath chloroplasts can take up, import and process the in vitro transcribed and translated LHCII precursor protein from L. gibba. Although bundle sheath chloroplasts incorporate LHCII into the pigmented light-harvesting complex, the efficiency is lower than that in mesophyll chloroplasts.
使用原位免疫定位和杂交技术，分别研究了玉米（Zea mays L.）中主要的捕光叶绿素 a/b 蛋白（LHCII）及其 mRNA 在鞘细胞和叶肉细胞中的分布。用玉米和浮萍的特异性 LHCII RNA 探针进行原位杂交，明确显示在鞘细胞和叶肉细胞中均存在高水平的 LHCII mRNA。使用 LHCII 单克隆抗体进行的原位免疫定位研究表明，两种细胞类型的叶绿体中均存在 LHCII 多肽。LHCII 的多肽组成和鞘细胞中的 LHCII 含量与叶肉细胞中的不同。叶肉和鞘细胞叶绿体都可以摄取、导入和加工来自浮萍的体外转录和翻译的 LHCII 前体蛋白。尽管鞘细胞叶绿体将 LHCII 掺入有色捕光复合物中，但效率低于叶肉叶绿体。
