Department of Biochemistry, Columbia University, New York, NY 10032.
Proc Natl Acad Sci U S A. 1985 Jan;82(2):386-90. doi: 10.1073/pnas.82.2.386.
The three-dimensional structure of a negatively stained hexagonal membrane lattice containing the light-harvesting chlorophyll a/b-protein complex and phospholipids has been determined to 30-A resolution by image reconstruction from electron micrographs. This lattice has p321 symmetry, a lattice constant of 125 A and a thickness of 75 A. The monomer is shown to be an elongated molecule about 65 A long in the dimension perpendicular to the plane of the membrane. It spans the hydrophobic domain of the membrane in an asymmetric fashion, projecting [unk]20 A from one surface and less from the other. On the basis of this image and available biochemical data, the structure of the complex in the native thylakoid membrane is proposed.
通过对电镜照片进行图像重建，我们已经将含有捕光叶绿素 a/b 蛋白复合物和磷脂的负染六方膜晶格的三维结构解析到 30-A 的分辨率。该晶格具有 p321 对称性，晶格常数为 125 A，厚度为 75 A。单体被证明是一个在垂直于膜平面的维度上约 65 A 长的拉长分子。它以不对称的方式跨越膜的疏水区，从一个表面伸出[unk]20 A，而从另一个表面伸出的较少。基于这个图像和现有的生化数据，提出了天然类囊体膜中复合物的结构。
